红曲黄色素亚慢性毒性研究  

Study on Subchronic Toxicity of Monascus Yellow Pigment

作　　者：陈冠敏[1,2] 林蔚[1,2] 林春芳[1,2] 黄宗锈[1,2] 郑丽红[1,2] 黄佳宁[1,2] 陈秀锦[1,2] 

机构地区：[1]福建省疾病预防控制中心 [2]福建省实验动物质量检测中心,福州350001

出　　处：《癌变．畸变．突变》2009年第4期316-319,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的:了解红曲黄色素对机体是否具有毒性作用。材料与方法:选用清洁级SD大鼠,试验设3个红曲黄色素剂量组(0.23、0.47、0.93 g/kg)及空白对照组(基础饲料)。试验期间观察动物形态,每周称重,记录饲料消耗量。第45 d时,采尾血测血常规、血生化;喂养90 d后,摘眼球采血作血常规及生化指标测定,然后取出肝、肾、脾、睾丸称重,并对肝、肾、脾、胃肠、睾丸、卵巢作病理学检查。结果:实验期间3个红曲黄色素组的大鼠体重增加、饲料利用率与对照组相比差异均无统计学意义(P>0.05)。喂养45 d和90 d后,3个红曲黄色素组大鼠的血红蛋白含量、红细胞、白细胞计数及白细胞分类均在正常值范围内。其血清谷丙转氨酶、谷草转氨酶、尿素、肌酐、总胆固醇、甘油三酯、血糖、总蛋白、白蛋白测定值均在正常值范围内。且3个红曲黄色素组大鼠的肾体比、脾体比、肝体比、睾体比与对照组比较差异均无统计学意义(P>0.05)。组织病理学检查结果显示各剂量组大鼠的肝、肾、胃、脾切片染色后在光镜下检查未见特异性病理改变。结论:在本实验条件下,红曲黄色素喂养90 d对大鼠未产生毒性作用,提示可作为天然色素在食品加工生产中在安全的剂量范围内可适当使用。BACKGROUND AND AIM： To investigate the toxicity in SD rats fed continuously with monascus yellow pigment. MATERIALS AND METHODS： SD rats were divided into four groups （0.23,0.47, 0.93 g/kg and a blank control） and fed continually for 90 days. During the experimental period, rats were weighed once every week, general conditions were assessed, and feed consumptions were recorded. On the 45^th day, blood was collected from tails to perform complete blood count and biochemical tests. After 90 days, eyeballs were extracted and blood was used for blood count and biochemical tests; livers, kidneys, spleens and testicles were weighed; and pathological examinations of livers, kidneys, spleens, stomach intestines, testicles and ovaries were done. RESULTS： The body weight increase and feed efficiency of all dose groups were not significantly different from those of control group（P 〉 0.05） .Complete blood count： the hemoglobin content, erythrocyte count, leukocyte count and differential in all dose groups were normal.Blood chemical test：serum ALT, AST, urea, creatinine, total cholesterol, triglyceride, glucose, total protein and albumin in all dose groups were normal. Organ/body weight ratios： the ratios of kidney/body, spleen/body, liver/body, testicle/body of all dose groups were not significantly different from those of control group（P 〉 0.05） .Pathological examination： no special were found in the livers, kidneys, stomachs and spleens of rats observed in rats continuously fed with monascus yellow pigment pigment in food production. pathological changes in all dose groups. CONCLUSION： No toxic effects were for 90 days, suggesting it could be used as a natural

关 键 词：红曲黄色素 血常规 血生化 体重 脏体比 毒性 

分 类 号：R155.5[医药卫生—营养与食品卫生学]