华蟾素对人乳腺癌细胞株MDA-MB-231生物学特性的影响  

Effect of cinobufacini on the biological characteristics of MDA-MB-231 breast cancer cell line

作　　者：殷文瑾 柳光宇 狄根红 吴炅 陆劲松 韩企夏 沈镇宙 刘鲁明[2] 邵志敏 

机构地区：[1]复旦大学附属肿瘤医院/肿瘤研究所乳腺外科复旦大学上海医学院肿瘤学系,上海200032 [2]复旦大学附属肿瘤医院中西医结合科,上海200032

出　　处：《肿瘤》2009年第7期641-644,共4页Tumor

摘　　要：目的:探讨华蟾素(cinobufacini)对人乳腺癌细胞株MDA-MB-231增殖、细胞周期和体外侵袭的影响及其可能机制。方法:用CCK-8试剂盒测定并绘制华蟾素作用后MDA-MB-231细胞的生长曲线,FCM法分析细胞周期分布,Transwell小室检测MDA-MB-231细胞的体外侵袭能力,RT-PCR检测细胞周期素(cyclin)及p21mRNA的表达变化。结果:华蟾素可抑制MDA-MB-231细胞的增殖能力,其半数抑制浓度(half inhibition concentration,IC50)为0.31mg/mL,抑制作用随着华蟾素作用时间的延长而增强,与对照组相比差异有统计学意义(P<0.05);Transwell小室检测表明,华蟾素作用后细胞的侵袭能力比对照组明显下降(P<0.05);FCM分析可见,随着华蟾素浓度的增加,停滞于S期的细胞比率比对照组明显增加(P<0.0001);华蟾素作用后,MDA-MB-231细胞cyclin A1、cyclin D1和cyclin E1 mRNA的表达水平下降,p21 mRNA的表达水平上升,但cyclin B1 mRNA的表达无明显改变。结论:华蟾素通过调控cyclin A1、cyclin D1、cyclin E1和p21的表达而抑制乳腺癌细胞的增殖和侵袭,并影响其细胞周期的分布。Objective： To investigate the effect of cinobufacini on proliferation, celly cycle distribution, invasion capability of MDA-MB-231 breast cancer cell line in vitro and possible mechanism. Methods： The effect of cinobufacini on cell growth was measured by CCK-8 reagent kit. Cell cycle distribution was determined by flow cytometry. The invasion capability in vitro was detected by Transwell chamber assay. The mRNA expressions of cell cycle related factors （cyclin） and p21 were tested by RT-PCR. Results： Cinobufacini inhibited proliferation of MDA-MB-231 cells. The half inhibition concentration （IC50） was 0.31 mg/mL. The inhibitory effect was time-dependent （P 〈0.05）. Cinobufacini significantly decreased invasion capability of MDA-MB-231 cells in vitro compared with control group （P〈0.05）. Cinobufacini induced S-phase arrest of MDA-MB-231 cells in a concentration-dependent manner （P〈0.0001）. Cinobufacini down-regulated the expression levels of cyclin A1, cyclin D1, and cyclin E1, while up-regulated that of p21 in MDA-MB-231 cell line. However, there was no marked change in the expression of cyclin B1. Conclusion： Cinobufacini inhibits cell proliferation and influences the cell cycle distribution in vitro by regulating the expression of cyclin A1, cyclin D1, cyclin E1 and p21 in breast cancer cells.

关 键 词：乳腺肿瘤 肿瘤侵润 细胞周期 华蟾素 细胞 MDA-MB-231 

分 类 号：R737.9[医药卫生—肿瘤]