麝香醒脑宁对大鼠全脑缺血/再灌注损伤的保护作用  

The protective effects of Shexiang Xingnaoning on global cerebral ischemia reperfusion injury

作　　者：汪洋[1,2] 李维祖[1] 尹艳艳[1] 曹曦[1] 明亮[1] 李卫平[1] 

机构地区：[1]安徽医科大学药理学教研室,合肥230032 [2]安徽中医药高等专科学校医疗系,芜湖241000

出　　处：《安徽医科大学学报》2009年第4期475-479,共5页Acta Universitatis Medicinalis Anhui

摘　　要：目的探讨麝香醒脑宁(SXN)对全脑缺血/再灌注损伤的保护作用及其机制。方法采用大鼠全脑缺血/再灌注模型,观察SXN对翻正反射和脑电恢复时间、脑含水量和脑指数、脑组织超氧化物歧化酶(SOD)、丙二醛(MDA)、乳酸脱氢酶(LDH)、乳酸(LD)、一氧化氮合酶(NOS)和海马内皮素(ET)的影响。结果SXN(80、160mg/kg)可促进全脑缺血/再灌注大鼠翻正反射和脑电活动的恢复;SXN(40、80、160mg/kg)能够降低全脑缺血/再灌注24h大鼠脑含水量和脑指数;SXN(80、160mg/kg)能够降低全脑缺血/再灌注大鼠脑组织MDA、LD含量,提高SOD、LDH活性,抑制脑组织NOS活性和海马ET的升高。结论SXN对全脑缺血/再灌注损伤具有一定的保护作用,其机制可能与抗自由基损伤、抑制NOS和ET的升高等有关。Objective To study the protective effects of Shexiang Xingnaoning（SXN） on global cerebral ischemia/ reperfusion injury and its mechanism. Methods The globe cerebral ischemia/reperfusion experiment induced by four vessels occlusion（4 VO）for 10rain and reperfusion for 24 h was performed to survey the influences of SXN on the recovery time of righting reflex and electrical activity of brain, the water content and cerebral index, the activity of SOD, LDH and NOS and the content of MDA, LD in brain tissue and ET in hippocampus. Results Compared with model group, SXN（80,160 mg/kg）significantly promoted the recovery of electroencephalogram and righting reflex. SXN（40,80,160 mg/kg）could reduce the brain edema and brain index obviously. SOD,LDH activities increased, NOS activity inhibited and MDA, LD content decreased in brain homogenate in rats treated by SXN （80, 160 mg/kg）. And SXN might be inhibited ET content in hippocampal homogenate in rats. Conclusion SXN takes protective effect on globe cerebral ischemia injury. The role of antioxidation, decreasing NOS activity and ET content might be contributed to its neuroprotective effects.

关 键 词：脑缺血 再灌注损伤 自由基 麝香醒脑宁 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R363[医药卫生—临床医学]