Wnt通路信号蛋白β-catenin和GSK-3β在孤独症模型大鼠脑中表达的变化  

Changes of the expression of β-catenin and GSK-3β in prefrontal cortex,hippocampus and cerebellum in an autism model of rat

作　　者：陈明军[1] 于剑锋[1] 柴继侠[1] 徐理[1] 王中平[1] 李瑞锡[1,2] 彭裕文[1] 

机构地区：[1]复旦大学上海医学院解剖与组织胚胎学系 [2]复旦大学脑科学研究院,上海200032

出　　处：《神经解剖学杂志》2009年第4期361-368,共8页Chinese Journal of Neuroanatomy

基　　金：复旦大学上海医学院基础临床交叉研究基金(No.2007-09);教育部博士点基金(No.070246184);复旦大学脑研究院开放课题基金(No.2007)资助项目

摘　　要：为探讨Wnt信号通路在孤独症发病中的作用,我们检测了Wnt信号通路中的两个关键信号蛋白分子β-catenin和GSK-3β在不同年龄阶段孤独症模型大鼠前额叶皮层,海马及小脑中的表达,同时对小脑进行了GSK-3β免疫组织化学染色检测。结果显示:孤独症模型动物的上述三个关键脑区内,β-catenin的表达水平显著升高,而GSK-3β的表达水平则明显降低;小脑内GSK-3β免疫反应阳性Purkinje细胞也明显减少。这些结果表明,孤独症模型大鼠脑内的Wnt信号通路信号传导亢进,而亢进的结果可能正是导致已知的孤独症脑内神经元发育异常的原因之一。由此提示:Wnt信号通路在孤独症的发病中起重要作用。In order to understand the role of the Wnt signaling pathway in the etiology of the autism, we examined the expressions of two signaling proteins,β-catenin and GSK-3β which take the key position in Wnt pathway, in the prefrontal cortex, hippocampus and cerebellum of an autism model of rat with different ages by means of the Western blot technique. And we also observed the GSK-3β immunoreactire neurons in the cerebellum by using immunohistochemical method. The results showed that the expression of the β-catenin was up-regulated, while the expression of the GSK-3β was decreased severely in the brain regions mentioned above in autism animal model. And the GSK-3β immunoreactive Purkinje cells in cerebellum were also reduced obviously. These results indicate that the Wnt signaling pathway is over activated in the autism models, and this activation might be the one of the causes of abnormal development of neurons in the brain of autism. Therefore, the present results suggest that Wnt signaling pathway plays an important role in the etiology of the autism.

关 键 词：Β-CATENIN GSK-3 β 孤独症动物模型 前额叶皮层 海马 小脑 大鼠 

分 类 号：R749.94[医药卫生—神经病学与精神病学]