血管内皮生长因子在海马伞-穹窿横断后隔区神经干细胞增殖分化中的作用  

Effects of vascular endothelial growth factor on proliferation and differentiation of neural stem cells after hippocampal fimbria-fornix transection

作　　者：马志健[1] 易西南[1] 李昌琪[2] 

机构地区：[1]海南医学院人体解剖学教研室,海南省海口市571101 [2]中南大学湘雅医学院人体解剖学及神经生物学系,湖南省长沙市410015

出　　处：《中国组织工程研究与临床康复》2009年第27期5276-5280,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：海南省自然科学基金资助项目(80440)~~

摘　　要：背景:目前已经证实成熟脑中存在神经干细胞,如何定向诱导其分化为某种特异性神经元从而替代损伤神经元有效治疗神经系统疾病非常关键。目的:探讨血管内皮生长因子对体内神经干细胞增殖和分化的作用。设计、时间及地点:随机对照动物实验,于2007-06/2008-06在中南大学湘雅医学院人体解剖学及神经生物学系完成。材料:健康雄性SD大鼠24只,随机分为模型对照组、抗体处理组、假手术组,8只/组。方法:模型对照组、抗体处理组大鼠建立海马伞-穹窿横断模型,假手术组大鼠仅做开颅手术。造模后即刻,抗体处理组吸取抗血管内皮生长因子抗体4μL,于损伤侧前囟+0.6mm,外侧+0.6mm,腹侧-5.5mm插入进针;模型对照组、假手术组注射等量生理盐水。主要观察指标:免疫组织化学法观察隔区血管内皮生长因子、巢蛋白、增殖细胞核抗原的表达,计算增殖细胞核抗原增殖指数。结果:与假手术组比较,模型对照组海马伞-穹窿横断后隔区血管内皮生长因子及巢蛋白的表达强度均明显增高(P<0.01);与模型对照组比较,抗体处理组巢蛋白表达强度明显降低(P<0.01)。假手术组增殖细胞核抗原在神经元胞浆内呈弥漫表达,表现为非特异性染色,增殖指数几乎为0;模型对照组出现少量增殖细胞核抗原阳性的神经元,增殖指数为1%;经抗体处理后增殖指数又降为0。结论:隔区损伤后血管内皮生长因子表达增加的同时确实有神经干细胞的出现。血管内皮生长因子的高表达可能是神经干细胞生成与分化的促进因素,是脑损伤后自我修复的基础。BACKGROUND： Mature brain contains neural stem cells. It is very important that how to directionally induce the differentiation of neural stem cells into a specific neuron, substitute for damaged neurons, in effective treatment of nervous system disease, OBJECTIVE： To study the role of vaSCular endothelial growth factor in the proliferation and differentiation of neural stem cells in vivo. DESIGN, TIME AND SETTING： The randomized controlled animal study was performed at the Department of Human Anatomy ＆ Neurobiology, Xiangya School of Medicine, Central South University from June 2007 to June 2008. MATERIALS： A total of 24 healthy male Sprague Dawley rats were randomly assigned to model control, antibody treatment and sham operation groups. METHODS： Rat models of hJppocampal fimbfia and fornix transsection were established in the model control and antibody treatment groups. Rats in the sham operation onty received digging skull operation. Immediately following model induction, rats in the antibody treatment group were subjected to 4 μ L anti-vascular endothelial growth factor antibody. The needle was inserted at anterior fontanelle＋0.6 mm, lateral side＋0.6 mm and ventral side-5.5 mm at the affected side. Rats in the model control and sham operation groups were subjected to an equal volume of saline. MAIN OUTCOME MEASURES： The expression of vascular endothelial growth factor, Nestin and proliferating cell nuclear antigen was observed using immunohistochemicat method. Proliferating cell nuclear antigen index was calculated. RESULTS： Compared with the sham operation group, the expression of vascular endothelial growth factor and Nestin was significantly increased at the septal area following hippocampal fimbda and fornix transsection in the model control group （P 〈 0.01）. Compared with the model control group, Nestin expression was significantly decreased in the antibody treatment group （P 〈 0.01）. The proliferating cell nuclear antigen diffusely expressed in neuronal cytoplasm in the

关 键 词：血管内皮生长因子 神经干细胞 穹隆海马伞横断 

分 类 号：R394.2[医药卫生—医学遗传学]