TAP1、TAP2和HLA-1在恶性黑素瘤组织中的表达  

Expression of transporters associated with antigen processing and HLA class I antigen in malignant melanoma

作　　者：李延[1] 陶娟[1] 刘业强[2] 杨井[1] 田分[1] 陆捷洁[1] 涂亚庭[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤科,武汉430022 [2]江西省九江市九江学院附属医院皮肤科,九江332000

出　　处：《中国麻风皮肤病杂志》2009年第7期487-489,共3页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金项目(30500437)

摘　　要：目的:检测抗原处理相关转运蛋白(transporters associated with antigen processing,TAP)和HLA-Ⅰ类分子在恶性黑素瘤组织中的表达。方法:应用免疫组织化学方法检测77例恶性黑素瘤组织TAP1、TAP2和HLA-Ⅰ类分子的表达,并进行半定量分析,以20例色素痣组织作为对照研究。结果:在77例恶性黑素瘤组织中,TAP1、TAP2和HLA-Ⅰ类分子的阳性表达率分别为23.38%、11.69%和63.64%,HLA-Ⅰ类分子的表达与TAP1和TAP2成正相关;而TAP1、TAP2和HLA-Ⅰ类分子几乎在所有的色素痣组织中都为阳性表达。结论:在恶性黑素瘤组织中TAP1、TAP2和HLA-Ⅰ类分子的表达水平下降,可能与人恶性黑素瘤细胞的免疫逃逸表型有关。TAP1和TAP2的表达下降可能是导致HLA-Ⅰ类分子表达下降的重要原因。Objective： To investigate the expression of TAP and HLA class I antigen in malignant melanoma （MM）. Methods： The expression of TAP and class- 1 antigen was measured by immunohistochemistry method and the correlation between the expression of TAP and class - 1 antigen was compared in 77 patients with MM. Samples from naevi of 20 cases were used as control. Results： Positive TAP1, TAP2 and HLA class I antigen immunostaining were observed in 23%, 12% and 64% of MM lesions respectively, and the expression of HLA class- I was positively correlated with that of TAP1 and TAP2. The expression of these molecules was positive in all of the pigmented naevi lesions. Conclusion： Our data suggest that reduced TAP1, TAP2 and HLA class - I antigen protein expression in MM may contribute to the immune escape phenotype of human melanoma cells, and the main cause of reduced HLA class - I expression may be due to the decreasing of TAP1 and TAP2 levels.

关 键 词：恶性黑色素瘤 TAP HIA—I类分子 

分 类 号：R739.5[医药卫生—肿瘤] R735.7[医药卫生—临床医学]