帕金森大鼠黑质和纹状体BMP4及其mRNA表达的时程变化  

Time Course Changes of Expression of BMP4 Protein and mRNA in Rat Models of Parkinson's Disease

作　　者：赵丹[1,2] 陈东风[2] 李伊为[2] 黎晖[2] 孟令杰[2] 周健洪[2] 邓汝东[2] 张瑞[2] 

机构地区：[1]海南医学院解剖学教研室,海南海口571101 [2]广州中医药大学解剖学教研室,广东广州510006

出　　处：《现代生物医学进展》2009年第13期2410-2412,2439,F0002,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金(NSFC30472272;NSFC30772861)

摘　　要：目的:探讨帕金森(PD)大鼠模型体内脑黑质和纹状体内骨形成蛋白4(BMP4)及其mRNA表达的变化规律。方法:用六羟基多巴胺(6-OHDA)建立PD模型后,第2、4、6、8和10周时处死大鼠,取左侧黑质、纹状体,用免疫组化、酶联免疫、荧光定量PCR技术从蛋白水平和基因水平检测BMP4及其mRNA表达。结果:BMP4及其mRNA表达基本一致,其BMP4及mRNA表达均呈双峰,其蛋白表达在纹状体内第2周和6周时达高峰、在黑质内第2周和8周时达高峰,其mRNA表达在纹状体内第2周和8周时达高峰、在黑质内第4周和8周达高峰,差异均有统计学意义(P<0.05)。结论:PD大鼠模型体内,BMP4及其mRNA不呈现稳定的低表达,而是有波动性,明确BMP4及其mRNA处于稳定低表达的时间后,为下一步的治疗实验时间点的选择上奠定了基础。Objective： To observe the expression of bone morphogenetic protein4 （BMP4） and mRNA of rat model of Parkinson＇s Disease（PD） induced by 6-OHDA with different times. Methods： Animal models of rats with 6-OHDA and different times （2,4,6,8,and 10 weeks） were established.The expressions of BMP4 and BMP4mRNA in substantia nigra and striatum were detected with ELISA and RT-PCR. Results： The expression of BMP4 and BMP4mRNA was similar between substantia nigra and striatum, expressing in double-peak, with statistical significance （P〈0.05）. Conclusions： The low expression of BMP4 and mRNA appears unstable after the estab- lishment of the rat model of PD;however, determination of the stable and low expression of BMP4 and mRNA will be conducive to the choice of the time of therapy for PD.

关 键 词：帕金森病 骨形成蛋白4 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]