山芹菜籽精油化学组成的GC-MS分析  

GC-MS Analysis of Essential Oil from Spuriopimpinella brachycarpa

作　　者：孙广仁[1] 刁绍起[1] 姚大地[1] 

机构地区：[1]北华大学,吉林132013

出　　处：《东北林业大学学报》2009年第7期102-103,共2页Journal of Northeast Forestry University

基　　金：吉林市科技局科研计划项目(吉市科农200716);北华大学科研项目(2006090号)资助

摘　　要：为了研究山芹菜(Spuriopimpinnella brachycarpa(Kom.)kitagawa)籽精油的化学组成,采用超临界CO2流体萃取法(SFE-CO2)与水蒸气蒸馏法(SDE)提取了山芹菜籽中的挥发油,采用气相色谱质谱联用仪(GC-MS)分别对超临界油与水蒸气油的挥发性成分进行了分析,同时对SFE-CO2油进行了甲酯化,采用GC-MS分析了脂肪酸组成。结果表明:从SFE-CO2油中分离出28种组分,保留时间4～17min;从SDE油中分离出16个组分,保留时间4～10min;对SFE-CO2油甲酯化后的分析结果表明,SFE-CO2油中含有棕榈酸、硬脂酸、油酸和亚油酸4种脂肪酸。表明SFE-CO2方法不仅能够萃取如脂肪酸乙酯类沸点较高的挥发性成分,还能提取难挥发的脂肪酸类化合物。An experiment was conducted to study the chemical composition of the essential oil from the seed of Spuriopimpinella brachycarpa by the methods of supercritical carbon dioxide fluid extraction （ SFE-CO2 ） and steam distillation extraction （SDE）. The chemical constituents were analyzed by means of capillary gas chromatography, combined with gas chromatography-mass spectrometry（GC-MS）. The essential oil extracted by SFE-CO2 was treated by methyl esterification and its fatty acid components were analyzed by GC-MS. Results showed that 28 constituents were identified from the essential oil by SFE-CO2 and the retention time ranged from 4 to 17 minutes, while 16 constituents were identified from the essential oil by SDE and the retention time between 4 and 10 minutes. The analysis of methyl esterification shows that the essential oil by SFE-CO2 contains four kinds of fatty acids, including palmitic acid, stearic acid, oleic acid, and linoleic acids. The method of SFE-CO2 could not only extract high boiling components including fatty-acid ethyl esters, but also extract some chemical components such as fatty acids, which are difficult to volatilize.

关 键 词：山芹菜籽 精油 超临界二氧化碳流体萃取 水蒸气蒸馏 GC—MS 

分 类 号：O657.63[理学—分析化学]