多棘海盘车多糖的提取及体外抑菌活性初探  

Extraction of polysaccharides from Asterias amurensis and preliminary study on their antibiotic activities in vitro

作　　者：周燕霞[1] 赵丽华[1] 邢翔[1] 张超[1] 

机构地区：[1]山东大学威海分校海洋学院,山东威海264209

出　　处：《中国海洋药物》2009年第3期58-60,共3页Chinese Journal of Marine Drugs

摘　　要：目的以威海沿海产多棘海盘车为原料,确定多糖的最佳提取工艺,并测定其抑菌活性。方法以苯酚-硫酸分光光度法测定多糖含量,考察酶解法,碱法,碱-酶结合等提取工艺的条件和优越性;采用滤纸片法测定海星多糖体外抑菌作用。结果氢氧化钠浓度为1mol·L-1,料液比为1∶2,提取2h时有最大产率。提取物对金黄色葡萄球菌有一定抑制作用。结论碱法优于其他制备方法;多棘海盘车粗多糖对细菌的抑制作用具有选择性。Objective To explore the extraction technology of polysaccharides from Asterias amurensis and study their antibacterial activities in vitro. Methods Determined by spectroscopic methodology with phenol - sulfuric acid, the polysaecharide content was taken as inspecting index to select the extraction technology such as enzymatic hydrolysis, alkali, and alkali-enzymatic combined. Filter paper method was used to test the polysaccharides antibiotic activities in vitro. Results The optimum extraction technology was adding 2 times amount of 1 mol · L^-1 sodium hydroxide, heating for 2h, under these conditions the greatest yield of starfish polysaccharides would be obtained which showed inhilitory effect on Staphylococcus aureus. Conclusion The alkal hydrolysis extraction technology is superior to the other methods; polysaccharides from Asterias amurensis showed antibiotic activity to some kinds of bacteria.

关 键 词：多棘海盘车 多糖 提取 抑菌活性 

分 类 号：TQ460.6[化学工程—制药化工] R965[医药卫生—药理学]