超临界CO2萃取灵芝子实体中麦角甾醇的实验研究  

Conditions for extraction of ergosterol from fruits of Ganoderma lucidum using supercritical fluid extration

作　　者：宋师花[1,2] 贾晓斌[1,3] 陈彦[1,3] 王丽静[2] 封亮[2] 

机构地区：[1]江苏省中医药研究院中药新型给药系统重点实验室,江苏南京210028 [2]江苏大学药学院,江苏镇江212013 [3]国家中医药管理局名医名方适宜剂型重点研究室,江苏南京210028

出　　处：《中国中药杂志》2009年第14期1783-1785,共3页China Journal of Chinese Materia Medica

基　　金：江苏省公益项目(BM2007701)

摘　　要：目的:探讨超临界CO2流体萃取灵芝子实体中麦角甾醇的可行性。方法:用HPLC测定麦角甾醇的含量,采用正交试验优化超临界CO2流体萃取灵芝子实体中麦角甾醇的工艺条件。结果:超临界CO2流体萃取麦角甾醇的最佳工艺条件:萃取压力20 MPa,萃取温度45℃,夹带剂(95%乙醇)用量为1.0 mL.g-1,萃取时间1.5 h。结论:超临界CO2流体具有极性可调的优势,可有效萃取灵芝中的麦角甾醇。Objective： To study the feasibility of supereritical fluid extraction （SFE） for extraction of ergosterols from the fruits of C, anoderma lucidum. Method： Optimum extraction conditions were studied by orthogonal tests, the ergosterol were analyzed by RP- HPLC. Result： The optimal extraction conditions were pressure as 20 MPa, temperature at 45 ~C, using ethanol as modifier carrier at the volume of 1 mL · g-1, and 1.5 hour of extraction time. Conclusion： SFE has the superiority of adjustable polarity, and has the ability of extracting ergosterol from fruits of Ganoderma lucidum.

关 键 词：灵芝 麦角甾醇 超临界CO2萃取 正交试验 HPLC 

分 类 号：TQ461[化学工程—制药化工]