Inhibitive Determination of Heavy Metal Ions by Conductometric Nitrate Reductase Biosensor  

作　　者：WANG Xue-jiang XIA Si-qing ZHAO Jian-fu ZHAO Hong-ning RENAULT Nicole Jaffrezic 

机构地区：[1]State Key Laboratory of Pollution Control and Resources Reuse, Tongji University, Shanghai 200092, P R. China [2]Laboratoire de Sciences Analytiques（UMR CNRS 5180）, Universite Claude Bernard Lyon 1, Universite ＇de Lyon, Villeurbanne Cedex 69622, France

出　　处：《Chemical Research in Chinese Universities》2009年第4期443-445,共3页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.20707014);the Opening Foundation of the Environmental Engineering Key Discipline,Zhejiang University of Technology,China(No.20080205)

摘　　要：A conductometric nitrate biosensor based on methyl viologen/Nafion/nitrate reductase（NR） interdigitated electrode for heavy metal determination was proposed.The activity of NR（EC 1.6.6.2） from Asper gillusniger（A.niger） was assayed as a function of metal concentration in the presence of Cu^2＋,Zn^2＋,Cd^2＋ and Pb^2＋.Parameters influencing the performance of the biosensor were optimized for the application of it in the inhibition determination of heavy metal ions.Detection limits for Cu^2＋,Zn^2＋,Cd^2＋ and Pb^2＋ were about 0.05,0.5,0.1 and 1.0 μmol/L,respectively.The results show that NR activity could not be regained after exposure to Cu^2＋,but could be partially recovered after exposure to Zn^2＋,Pb^2＋ and Cd^2＋.A conductometric nitrate biosensor based on methyl viologen/Nafion/nitrate reductase（NR） interdigitated electrode for heavy metal determination was proposed.The activity of NR（EC 1.6.6.2） from Asper gillusniger（A.niger） was assayed as a function of metal concentration in the presence of Cu^2＋,Zn^2＋,Cd^2＋ and Pb^2＋.Parameters influencing the performance of the biosensor were optimized for the application of it in the inhibition determination of heavy metal ions.Detection limits for Cu^2＋,Zn^2＋,Cd^2＋ and Pb^2＋ were about 0.05,0.5,0.1 and 1.0 μmol/L,respectively.The results show that NR activity could not be regained after exposure to Cu^2＋,but could be partially recovered after exposure to Zn^2＋,Pb^2＋ and Cd^2＋.

关 键 词：Conductometric biosensor Heavy metal Nitrate reductase 

分 类 号：S512.101[农业科学—作物学] TS207.51[轻工技术与工程—食品科学]