乳凝集素对轮状病毒感染新生大鼠肠道分泌IL-2、IL-4及INF-γ的影响  

Effect of lactadherin on IL-2、IL4 and INF-γ secreted by intestinal tract of newborn rats with rotavirus infection

作　　者：杨花梅[1] 高娟[1] 盛慧英[2] 周一珺[1] 陈同辛[1] 朱建幸[1] 何振娟[1] 

机构地区：[1]上海交通大学医学院附属新华医院儿内科,200092 [2]广州市儿童医院,510120

出　　处：《国际儿科学杂志》2009年第4期328-330,共3页International Journal of Pediatrics

基　　金：国家自然科学基金资助项目（30571979）

摘　　要：目的探讨轮状病毒感染新生SD大鼠肠道分泌白细胞介素（IL）-2、IL4及γ干扰素（INF-γ）浓度的变化及乳凝集素（laetadherin）对其的影响。方法10日龄清洁级SD大鼠45只，随机分为正常对照组（N组）、轮状病毒感染组（V组）和轮状病毒感染＋laetadhefin组（VL组）三组，每组15只。N组予以人工喂养；V组在人工喂养的第1天同时给予轮状病毒喂养；VL组人工喂养的第1天给予轮状病毒喂养，出现腹泻症状后给予每天0．25mg lactadhefin喂养，直到腹泻停止。分别于给病毒前（T1）、腹泻最严重时（T2）及腹泻结束后（T3）各时间点取肠内容物上清，测定细胞因子IL-2、IL-4和IFN-γ浓度。结果三组大鼠小肠内IL-2、IL4和IFN-γ浓度在T1时间点差异元明显统计学意义；在T2时间点，V组和VL组IL-2、INF-γ浓度分别为（97．97±7．99）、（25．00±8．32）pg／ml和（70．00±8．27）、（20．53±2．10）pg/ml，均低于N组[（348．84±45．44）、（33．95±5．76）pg/ml]（P均〈0．05）；而在T3时间点，IL组IL-2、INF-γ浓度明显高于V组[（369．83±36．83）、（42．59±7．54）pg/ml与（293．59±45．64）、（31．35±3．63）pg／ml，P〈0．05]，VL组与N组浓度差异无明显统计学意义。结论Lactadhefin在轮状病毒感染大鼠能提高IL-2和IFN-γ浓度，在抗轮状病毒感染中有一定的积极作用。Objective To explore the influence of lactadherin on gut contents level of IL-2. IL-4 and IFN-γ in newborn rats with rotavirus infection.Methods Forty-five ten-day-old SD rats were randomly divided into three groups： control group（group N）, rotavirus infection group（group V）, rotavirus infection and lactadherin group（group VL）. All the rats were fed with formula,group V and group VL were fed with rotavirus（ 1 × 10^6） on the first day,then group VL were fed 25 mg/d lactadherin when they started diarrhea, until recovery. Gut contents samples were taken before feeding（T1）, with most serious diarrhea（T2）,after recovery（T3）,the level of IL-2,IL-4 and IFN-γ in the gut contents were detected by ELISA. Results At time of T1 gut contents level of IL-2.IL-4 and IFN-γ were no statistically significant differences. Gut contents level of IL-2 and INF-γ were higher in group N[ （ 348.84 ± 45.44） pg/ml, （ 33.95 ± 5.76） pg/ml] than those in group V[ （97.97 ± 7.22） pg/ml, （25.00 ± 8.32） pg/ml] and group VL[ （70.00 ± 8.27） pg/ml, （20.53 ± 2.10） pg/ml] at time of T2（ P 〈 0.05 ）. At time of T3 gut contents level of IL-2 and INF-3, were higher in group VL than those in group V[ （369.83 ± 36.83）pg/ml, （42.59 ± 7.54） pg/ml vs. （293.59 ± 45.64） pg/ml, （31.35 ± 3.63） pg/ml, P 〈 0.05],but the differences between group VL and group N were not statistically significant. Conclusions Lactadherin could boost gut contents level of IL-2 and INF-γ in rats with mtavirus inretctiom,it plays a certain positive role on antimtavirus infection.

关 键 词：LACTADHERIN 细胞因子 轮状病毒 新生大鼠 

分 类 号：R725.1[医药卫生—儿科]