P16^(INK4α)及Ki67在正常宫颈、慢性宫颈炎及宫颈上皮内瘤变中的表达及意义  

EXPRESSION AND SIGNIFICANCE OF P16^(INK4α) AND Ki67 IN THE NORMAL CERVIX,CHRONIC CERVICITIS AND CERVICAL INTRAEPITHELIAL NEOPLASIA

作　　者：陈瑛[1] 黄磊[1] 袁静萍[2] 张庆华[1] 敖启林[3] 

机构地区：[1]湖北省武汉市中心医院妇产科,武汉430014 [2]湖北省武汉市中心医院病理科,武汉430014 [3]华中科技大学同济医学院附属同济医院病理研究所,同济医学院病理学系,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2009年第4期426-430,共5页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的探讨联合检测P16INK4α、Ki67的表达在子宫颈上皮内瘤变(CIN)中的诊断价值。方法用免疫组化S-P法对10例正常宫颈、20例慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ各30例中P16INK4α和Ki67的表达进行检测。结果P16INK4α在CIN的阳性表达明显高于与正常宫颈和宫颈慢性炎症组织中的表达,差异有显著性意义(P<0.05)。ki67在CINⅠ、CINⅡ、CINⅢ的表达三者比较差异有显著性意义(P<0.05)。结论P16INK4α在判断是否有宫颈上皮内瘤变(CIN)时具有很高的特异性和敏感性,结合Ki67标记可以准确判断CIN级别。联合检测P16INK4α和Ki67可以作为判断慢性宫颈炎与CIN的客观指标。Objective To explore the diagnostic value of tne combineo aetecuon of P16^INK4α in cervical intraepithelial neoplasia. Methods The expression of P16^INK4α and Ki67 were measured by S-P immnohistochemistry, respectively, in 10 case of normal cervical tissues, 20 chronic cervicitis, 30 CIN Ⅰ, 30 CIN Ⅱ and 30 CINⅢ. Results The expression of P16^INK4α in CIN was remarkably higher than that in normal cervical tissues and chronic eervicitis （P〈0.05） . The expression of ki67 was notably different in CIN Ⅰ, CIN Ⅱ and CINⅢ （P〈0.05） . Conclusion The detection of P16^INK4α expression is highly specific and sensible in judging cervical intraepithelial neoplasia, while the detection of Ki67 can accurately determine the grade of CIN. The combined detection of P16^INK4α and Ki67 is recommended to be an objective indicator in defining the chronic cervicitis and CIN.

关 键 词：P16INK4α KI67 免疫组化 子宫颈上皮内瘤变 诊断 

分 类 号：R361[医药卫生—病理学]