人脐带间充质干细胞兔眼玻璃体腔注射的安全性研究  

Injection of human umbilical cord derived mesenchymal stem cells into the vitreous of rabbits

作　　者：孙国玲[1] 汪建涛[1] 孟恒星 吴建国[1] 李筱荣[1] 

机构地区：[1]天津医科大学眼科中心,300070 [2]协和干细胞基因工程有限公司

出　　处：《中华眼底病杂志》2009年第4期257-260,共4页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的观察人脐带问充质干细胞（hUC—MSCs）兔眼玻璃体腔注射后的生存情况及安全性。方法27只青紫蓝兔随机分为玻璃体腔注射1、2、4周组，每组9只兔。右眼为hUC—MSCs玻璃体腔注射组（实验组），左眼为培养液玻璃体腔注射组（对照组）。注射前后分别采用裂隙灯显微镜进行眼前节检查，间接检眼镜、眼底照相、荧光素眼底血管造影（FFA）进行眼底检查，Tono—pen眼压计测量眼压。体外碳花氰荧光染料CM—Dil荧光标记hUC—MSCs。兔眼玻璃体腔hUC—MSCs注射后4周，共聚焦荧光显微镜下观察hUC—MSCs在体内的存活情况，然后摘除眼球行光学显微镜和透射电子显微镜检查。结果hUC-MSCs玻璃体腔注射后4周，大量hUC—MSCs仍旧存活于玻璃体腔，结构完整。兔全身情况良好；裂隙灯显微镜检查显示眼前节无明显改变；间接检眼镜和眼底照相检查显示实验眼和对照眼视网膜无明显改变；FFA检查显示注射后1、2、4周视网膜血管无异常荧光和荧光渗漏；注射前后各时间点眼压差异无统计学意义（t=0．125，P〉0．05）；光学显微镜和透射电子显微镜检查显示兔眼角膜、房角、晶状体、视网膜结构无明显变化。结论hUC—MSCs在玻璃体腔注射后4周仍存活于体内；hUC—MSCs玻璃体腔注射安全可行。Objective To observe the survival of human umbilical cord derived mesenchymal stem cells （hUC-MSCs） after injection into the vitreous of rabbits, and the animal safety under those procedures. Methods Twenty-seven pigmented rabbits were randomly divided into 3 groups （intravitreat injection 1 week group,2 weeks group and 4 weeks group）, each with 9 rabbits. For each animal the right eye was the experimental eye receiving hUC-MSCs injection, while the left eye was the control eye receiving culture medium. The rabbit eyes were examined by slit-lamp microscope, indirect ophthalmoscopy, fundus photography, fundus fluorescence angiography（FFA）and Tono-pen tonometer before and after injection. hUC-MSCs were labeled by CM-Dil in vitro, and their survival status was measured by confocal fluorescence microscopy, light microscope and transmission electron microscope at 4 weeks after injection. Results Four weeks after injection, a large number of the hUC-MSCs were still alive in the vitreous cavity. The overall condition of those rabbits was good. The anterior segment and retina of experimental eyes were normal, without hyperfluorescence, hypofluorescence and leakage in the retina at 1, 2 and 4 weeks after injection. There was no significant difference on IOP before and after injection at different time points （P^0.05）, and no obvious changes at cornea, anterior chamber angle, lens, retinal structure by. light microscope and transmission electron microscope examination. Conclusion hUC-MSCs can survive in the rabbit vitreous for four weeks;intravitreal injection of hUC-MSCs was safe and feasible.

关 键 词：干细胞/细胞学 脐血干细胞移植/副作用 注射/方法 动物 实验 

分 类 号：R770.5[医药卫生—眼科]