兔视网膜下非活动载片植入后视网膜免疫组织化学观察  

Immunohistologicai observation on rabbits＇ retinae after subreinal implantation with inactive chips

作　　者：刘芳[1] 刘武[1] 卢清君[1] 李彬[1] 李辽青[1] 莫宾[1] 李倩[1] 

机构地区：[1]首都医科大学附属北京同仁医院眼科中心,100730

出　　处：《中华眼底病杂志》2009年第4期292-296,共5页Chinese Journal of Ocular Fundus Diseases

基　　金：基金项目：国家自然科学基金（30571989）

摘　　要：目的观察兔视网膜下非活动载片植入后视网膜相关蛋白的表达。方法健康成年新西兰白兔27只，随机分为手术组、伪手术组及对照组，3组分别为12、12、3只兔。手术组和伪手术组再分为手术后7、15、30、60d亚组，每组随机分配3只兔，手术组为非活动载片植入，伪手术组非活动载片植入随即取出。于手术后7、15、30、60d处死动物后进行免疫组织化学染色，对照组于饲养30d后处死动物行免疫组织化学染色，观察胶质纤维酸性蛋白（GFAP）和脑源性神经营养因子（BDNF）在视网膜的表达。结果手术组手术后7、15、30d组载片上外层视网膜明显变薄，内层细胞排列紊乱；60d组胶质细胞增生，手术组载片上视网膜GFAP和BDNF阳性表达均较伪手术组和对照组增加，伪手术组GFAP和BDNF阳性表达均较对照组增加，各组内各时间点间GFAP和BDNF表达未见明显差异。结论载片植入后局部视网膜神经保护相关蛋白表达升高，提示载片植入可能有神经保护作用参与，但其作用有限。Objective To observe the expression of related proteins of retina after subretinal implantation with inactive chips. Methods A total of 27 healthy adult New Zealand white rabbits were randomly divided into three groups： operation group （12 rabbits） in which the rabbits were implanted with inactive chips into the interspace beneath retina; sham-operation group （12 rabbits） in which the rabbits were implanted with inactive chips into the interspace beneath retina which was taken out immediately; the control group （3 rabbits）. Animals were saerified for immunohistological study 7, 15, 30 and 60 days after surgery. The rabbits in control group group were sacrified for immunohistologica] study after bred for 30 days. The expressions of glial fibrillary acidic protein （GFAP） and brain derived neurotrophie facor （BDNF） were observed. Results In operation group, the outer nulear layer of retina thinned, and the cells in the inner nulear layer was disorganized 7, 15, and 30 days after the surgery; glial cells proliferated 60 days after surgery; the positive expression of BDNF and GFAP was more than that in the sham-operation and control group. In sham-operation group, the positive expression of BDNF and GFAP was more than that in the control group. No obvious difference of expression of BDNF and GFAP between each time point groups was found. Conclusions The＇expression of neroprotective related proteins increased after subretinal implantation with inactive chips suggests that limited neuroprotective effects might be led by the implantation.

关 键 词：神经胶质原纤维酸性蛋白质/免疫学 脑源性神经营养因子/免疫学 免疫组织化学/方法 植入物 实验性/副作用 动物 实验 

分 类 号：R774[医药卫生—眼科]