应用液压冲击颅脑损伤仪建立大鼠外伤性视神经损伤动物模型  

Optic nerve injury model in rats set up by fluid percussion brain injury device

作　　者：颜华[1] 张琳琳[1] 李凤荣[2] 

机构地区：[1]天津医科大学总医院眼科,300052 [2]天津医科大学生物学教研室

出　　处：《中华眼底病杂志》2009年第4期301-305,共5页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的观察应用液压冲击颅脑损伤仪（FPI）能否成功建立大鼠外伤性视神经损伤动物模型。方法成年雌性Wister大鼠71只，随机选取5只为正常对照组，其余66只为模型组。模型组再随机分为3组。第1组8只大鼠，分别于损伤前、损伤后1、3d、1、2、4、6、8周行双眼闪光视觉诱发电位（F—VEP）及视神经核磁共振成像（MRI）检查；第2组56只大鼠，随机分成7个亚组，每个亚组8只大鼠，分别于损伤后1、3d、1、2、4、6、8周行视网膜组织病理学及末端脱氧核苷酸移换酶介导的dUTP缺口末端标记测定法（TUNEI．）检测；第3组2只大鼠，分别于损伤后4、8周行视神经透射电子显微镜检测。根据打击力量不同，将损伤眼分为轻、重2组。重伤组打击锤以25。的预定角度打击视神经，平均打击力量（699．14±60．79）kPa，轻伤组以15。的预定角度打击，平均打击力量（243．18±20．26）kPa。每只大鼠右眼为重伤组，左眼为轻伤组。结果重伤组损伤后ld主波潜伏期延长，与正常对照组比较，差异有统计学意义（t=2．06，P〈0．05）；FVEP振幅在损伤后2周内逐渐降低，2周后趋于稳定（F=1．98，P〉0．05）。轻伤组损伤后1d主波潜伏期延长，与正常对照组比较，差异有统计学意义（t=2．19，P％0．05）；振幅在损伤后4周内逐渐降低，4周后趋于稳定（F=1．62，P〉O．05）。MRI检查显示，损伤后1d视神经高信号，损伤后8周仍较明显。组织病理学检查发现，损伤后1d视网膜神经节细胞层（GCL）可见毛细血管破裂出血；损伤后4周GCL内可见空化的视网膜神经节细胞。损伤后3d视网膜各层均出现凋亡阳性细胞；TUNEL染色发现损伤后1、2周凋亡阳性细胞明显增多。结论应用FPI能成功建立大鼠外伤性视神经损伤模型。Objective To observe whether the animal model of optic nerve injury in rats can be set up by fluid percussion brain injury device （FPI） or not. Methods Seventy-one healthy female Wister rats were randomly divided into 2 groups, inlcuding model group with 66 rats and control group with 5 rats. The rats in model group were randomly divided into 3 groups. Eight rats in group 1 were examined by flash-visual evoked potential （F-VEP） and magnetic resonance imaging （MRI） examines before and 1, 3 days, 1, 2, 4, 6, and 8 weeks after injury; 56 rats in group 2 were randomly divided into 7 subgroups with 8 rats in each subgroup,and were detected by histopathological and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） apoptosis examines 1, 3 days, 1, 2, 4, 6, 8 weeks after injury;2 rats in group 3 were examined by electron microscopy 4 and 8 weeks after injury. According to the degree of injury, the injured eyes were divided into 2 groups including severe injury group with the beat pressure of （699. 14±60.79） kPa and mild injury group with the beat pressure of （243.18±20.26） kPa. The right and left eyes in rats in each group were in severe and mild injury group, respectively. Results One day after injury, the latency duration of F-VEP prolonged in severe injury group, with differed much form which in the normal control group （P〈0.05） ;the amplitude was gradually reduced during the first 2 weeks after injury and kept steady after that （P〉0.05）. The latency duration prolonged in mild injury group,and its difference with the normal control group was statistically significant （P〈0.05） ;the amplitude was gradually reduced during the first 4 weeks after injury and kept steady after that （P〈0.05）. The abnormal high signal could be seen onoptic nerve 1 day after injury, and was still obvious 8 weeks later. The results of histopathologlcal examination showed ruptured capillary in ganglion cell layer 1 day after injury; retinal ganglion cells without nucle

关 键 词：颅脑损伤/并发症 视神经损伤/外科学 模型 动物 

分 类 号：R779.1[医药卫生—眼科] R-332[医药卫生—临床医学]