壳寡糖和双歧杆菌对腹水型肝癌细胞H22的抑制作用  

Inhibitive effect of chitooligosaccharides and bifidobacterium on ascites H_(22) hepatoma

作　　者：曹维强[1,2] 赵海田[3] 杨鑫[3] 佘永新[1] 王静[1] 

机构地区：[1]中国农业科学院农业质量标准与检测技术研究所,北京100081 [2]中国惠州出入境检验检疫局综合技术中心,广东惠州516006 [3]哈尔滨工业大学食品科学与工程学院,哈尔滨150090

出　　处：《哈尔滨工业大学学报》2009年第7期241-244,共4页Journal of Harbin Institute of Technology

基　　金：黑龙江省自然科学基金资助项目(B0309)

摘　　要：采用过氧化氢氧化法制备水溶性壳寡糖,采用Q TRAP LC/MS/MS System测定壳寡糖的平均分子量,观察壳寡糖和双歧杆菌对腹水型肝癌细胞H22的抑制作用.使用JEM1200EX透射电子显微镜观察不同浓度壳寡糖和双歧杆菌对H22细胞作用后H22细胞学的变化,结果表明,平均分子量为609的壳寡糖及双歧杆菌单独对腹水型肝癌细胞H22抗(抑)制作用均显著;壳寡糖(浓度为1.5%)与双歧杆菌(109CFU/mL)具有协同增效抗肿瘤作用.Chitooligosaccharides were prepared by hydrogen peroxide method. The Q-TRAP LC/MS/MS System was employed to determine the average molecular weight of chitooligosaceharides and study the inhibitive effect of chitooligosaceharides and bifidobacterium on ascites H22 hepatoma. The cytology changes of H22 effected by chitooligosaccharides and bifidobacterium with different concentrations were observed by JEM1200EX electron microscope. The result show that chitooligosaccharides with average molecular weight of 609 and bifidobacterium have obviously inhibitive effect on aseites H22 hepatoma respectively, and the effect can be enhanced with the combination of ehitooligosaeeharides with concentration of 1.5% and bifidobacterium of 10^9 CFU/mL.

关 键 词：壳寡糖 双歧杆菌 腹水型肝癌细胞H22 抗(抑)制作用 

分 类 号：TS201.2[轻工技术与工程—食品科学]