主动免疫治疗中迟发型超敏反应的初步研究  

Analysis of delayed-type hypersensitivity (DTH) in active specific immunotherapy (ASI) for patient with advanced malignant tumor

作　　者：白东晓[1] 王月[1] 肖建安[1] 魏林生[1] 

机构地区：[1]河南省安阳市肿瘤医院外一科,455000

出　　处：《中国实用医药》2009年第22期6-7,共2页China Practical Medicine

摘　　要：目的探讨新城鸡瘟病毒修饰的自体肿瘤疫苗(NDV-ATV)主动免疫治疗(ASI)前后机体的细胞免疫功能变化。方法对32例术后的进展期恶性肿瘤患者,在NDV-ATV接种前及第4次接种后一周,检测机体对肿瘤抗原的迟发型超敏反应(DTH)反应,每个患者均以PPD作为对照。接种前后,在12例患者DTH反应部位取皮肤活检,Envision二步法免疫组化染色,检测致敏的CD8+T细胞、CD68+巨噬细胞数。抽取外周血检测接种前后的CD4+T细胞及CD8+T细胞的变化。结果肿瘤抗原的DTH值,主动免疫治疗后明显大于接种前;免疫治疗后外周血CD4+T细胞及CD8+T细胞比例明显升高;免疫治疗后DTH部位真皮组织内CD8+T细胞、CD68+巨噬细胞数目明显增多。结论NDV-ATV能够改善机体的细胞免疫功能。Objective To evaluate the immunological function of patients with advanced malignant tumor after active specific immunotherapy using new castle disease virus-modified autologous tumor vaccine （NDV-ATV）. Methods Thirty-two post operative patients with advanced malignant tumor were enrolled in this study, before ASI and after the fourth innoculation, we injected sepecfic tumor antigen and purified protein deriv- ative（PPD） in homologous hypoderma as a control to detect the value of delayed-type hypersensitivity（DTH） and the erythema and induration value initiated by PPD. Among the 12 patients, we got the skin tissue of DTH site to make Envision immuno-histochemistry staining so as to count the number of sensitized CD8＋ Tcells and CD68 ＋ macrophages before ASI and after the fourth inoculation. Intracellular cytokine of CD4 ＋ Tcell and CD8＋Tcell in PBMNC were detected by flow cytometry before and after the inoculation. Results DTH numerical value in- creased after ASI. After ASI,The serum level of CD4＋ T cell and CD8＋ Tcell was elevated obviously. The number of sensitized CD8 ＋ Tcells and CD68＋ maerophages in the DTH site was enlarged. Conclusion Inoculation with NDV-ATV can improve the cell-mediated immunological function of tumor patients.

关 键 词：主动免疫治疗 迟发型超敏反应 

分 类 号：R730.51[医药卫生—肿瘤]