酒精联合高脂饮食对大鼠脂肪组织磷脂酰肌醇3激酶表达的影响  

Effects of alcohol and high-fat diet on the expression of phosphatidylinositol 3-kinese in rat adipose tissue

作　　者：于东[1] 彭晓琳[1] 赵立娜[1] 田利钺[1] 应晨江[1] 郝丽萍[1] 杨雪峰[1] 孙秀发[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院营养与食品卫生学系,武汉430030

出　　处：《卫生研究》2009年第4期395-397,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30300282)

摘　　要：目的研究酒精和高脂饮食对雄性大鼠脂肪组织磷脂酰肌醇3激酶(PI-3K)P85αmRNA及蛋白水平表达的影响。方法酒精灌胃和高脂饮食方法建立动物模型。测定空腹血糖和血胰岛素,计算胰岛素抵抗指数(HOMA-IR)。通过RT-PCR和Western blotting方法测定P85αmRNA及蛋白水平。结果与普通对照组相比,高脂对照组HOMA-IR指数升高;与高脂对照组相比,各剂量酒精和高脂饮食组血糖升高,胰岛素抵抗指数在低中剂量组升高,各剂量酒精联合高脂饮食组脂肪组织PI-3KP85αmRNA及蛋白表达水平均降低(P<0.05)。结论高脂饮食导致胰岛素抵抗;与单独高脂饮食相比,酒精联合高脂饮食加剧胰岛素抵抗程度。磷脂酰肌醇3激酶P85α mRNA及蛋白水平改变。Objective To investigate the effects of alcohol and high-fat diet on the expression of PI-3K-P85α in rat adipose tissue, and in order to explore the effects on insulin sensitivity and the molecular mechanisms induced by combination of alcohol and high-fat diet. Methods The rats were treated by alcohol and high-fat. Then the contents of fasting plasma glucose and insulin, and HOMA-IR index of each group were calculated. Expression of PI-3K-P85α were detectedby RT-PCR and Western blotting. Results In comparison with normal diet group, HOMA-IR index of high-fat groups increased, in comparison with high-fat control group, the contents of fasting plasma glucose increased in alcohol combined with high-fat groups, and HOMA-IR increased both in the low-dose and middle-dose alcohol combined with highfat model groups. Expressions of PI-3K-P85α remarkably decreased in all the alcohol and high-fat model groups（ P 〈 0. 05）. Conclusion High-fat diet could induce insulin resistance. Compared with high-fat diet alone, alcohol combined with high-fat could aggravate insulin resistance. The changes of PI-3K-P85α expression in adipose tissue may be one of the molecular mechanism of insulin resistance induced by alcohol combined high-fat diet.

关 键 词：胰岛素抵抗指数 磷脂酰肌醇3激酶 胰岛素抵抗 酒精 高脂饮食 

分 类 号：R587[医药卫生—内分泌] R155[医药卫生—内科学]