大鼠灌胃槲皮素后门静脉槲皮素及其代谢产物的液质分析  

HPLC-MS analysis of quercetin and its metabolites in portal vein after intragastrical administration of quercetin in rats

作　　者：安代志[1] 吴胜明[2] 韦京豫[1] 杨继军[1] 董方霆[2] 郭长江[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050 [2]国家生物医学分析中心

出　　处：《卫生研究》2009年第4期417-419,共3页Journal of Hygiene Research

基　　金：国家自然科学基金项目(No.30571567)

摘　　要：目的分析经口给予槲皮素后大鼠门静脉血槲皮素及其代谢产物。方法健康雄性Wistar大鼠按40mg/kg剂量经口给予槲皮素1.0小时后采集门静脉血液制备血浆,采用高效液相-质谱联用的方法进行分析。结果槲皮素处理大鼠门静脉血中可以检测到一定浓度的槲皮素以及异鼠李素、怪柳黄素、槲皮素硫酸酯、槲皮素-3-葡萄糖醛酸、槲皮素葡萄糖醛酸化硫酸酯。结论经口给予槲皮素经肠吸收代谢后以多种形式进入体内。Objective To analyse the plasma contents of querceitn and its metabolites in portal vein in rats. Methods Male Wistar rats were orally administrated with quereetin at the dose of 40mg/kg. The blood samples were corrected from the portal vein l h later. The quereetin and its metabolites in plasma were analysed with high performance liquid chromatography-mass spetrometry （HPLC-MS） method. Results The quercetin, quercetin-3-glueuronide, quereetin glucuronide sulfate, 3＇-O-methylated quercetin, 4＇-O- methylated quercetin and quercetin sulfate were detected in portal vein. Conclusion The results indicated that the quereetin was extensively metabolized in intestine and entered the portal vein in various forms after administration.

关 键 词：槲皮素 高效液相色谱-质谱分析 大鼠 

分 类 号：R151.3[医药卫生—营养与食品卫生学]