乳腺癌中胰岛素样生长因子2印迹状态的研究  

Study on genomic imprinting of IGF2 in breast cancer

作　　者：杨蓉[1] 王友洁[2] 朱晓玲[2] 高晓晖[2] 孟利平[2] 吴韶彬[2] 吴宇[2] 杜玉开[2] 

机构地区：[1]武汉市妇女儿童医疗保健中心,武汉430016 [2]华中科技大学同济医学院公共卫生学院儿少卫生与妇幼保健学系

出　　处：《卫生研究》2009年第4期423-425,共3页Journal of Hygiene Research

基　　金：国家自然基金项目资助(No.30400358)

摘　　要：目的探讨乳腺癌中胰岛素样生长因子-2(IGF-2)的印迹状态与乳腺癌发生发展的关系。方法采用聚合酶链反应(PCR)和限制性片断长度多态性(RFLP)的方法,在47例乳腺癌组织中筛选IGF-2杂合标本,并用逆转录PCR-RFLP检测杂合标本IGF-2的印迹状态。结果在癌组织和癌旁组织中都发现了IGF2的印迹丢失,癌旁组织中IGF2的印迹丢失率(79.2%)比癌组织中的发生率(50.0%)高,两组的差异有统计学意义(P=0.035)。如果乳腺癌组织中发现有IGF-2印迹丢失,其对应的癌旁组织也发现了IGF-2的印迹丢失,但如果乳腺癌组织中为IGF-2印迹保持,则其对应的癌旁组织有的为IGF-2印迹保持,有的为IGF-2印迹丢失。结论IGF2的印迹丢失可能参与了乳腺癌的发展过程。Objective To explore the imprinting status of the insulin like growth factor-2 associated with the development of breast cancer. Methods The samples of heterozygotes of IGF-2 was identified by polymerase chain reaction （PCR） and restriction fragment length polymorphism （RFLP） in 47 breast cancers tissue and their adjacent tissues, and then imprinting status of IGF-2 in these heterozygosis were detected by RT-PCR-RFLP. Result Losses of imprinting of IGF2 were found in both of the cancer tissues and adjacent normal breast tissue and the incidence rates of losses of printing in the adjacent tissues（79.2% ） were more higher than those in the cancer tissues（50.0% ）, it has statistical significance （ P = 0.035）. If there were losses of imprinting of IGF-2 in the breast cancer tissues, then there were Losses of imprinting of IGF-2 in their corresponding adjacent tissues. But If there were maintain of imprinting of IGF- 2 in the breast cancer tissues, then there were losses and maintain of imprinting of IGF-2 in their corresponding adjacent tissues. Conclusion The loss of imprinting of IGF-2 could be involved in the development of breast cancer.

关 键 词：乳腺癌 胰岛素样生长因子-2 印迹状态 

分 类 号：R737.1[医药卫生—肿瘤] R730.231[医药卫生—临床医学]