井冈霉素菌种自然选育及产素能力研究  

Studies on the Natural Screen of Validamycin Strain and its Producing Capability

作　　者：钟世华[1] 齐风佩[1] 范明亮[1] 徐满才[1] 

机构地区：[1]湖南师范大学化学化工学院,中国长沙410081

出　　处：《湖南师范大学自然科学学报》2009年第2期85-88,共4页Journal of Natural Science of Hunan Normal University

基　　金：国家自然科学基金资助项目(20602010)

摘　　要：采用自然选育方法对井冈霉素生产菌株(Streptomyces hygroscopicus var.Jinggangensis Yen)进行筛选,获得了遗传性能稳定的高产菌株,考察了碳源、有机氮源对菌种产素能力的影响.结果表明:采用C∶N为1∶0.45～1∶0.57,固形物含量在12%左右的培养基配方,井冈霉素生产菌产素能力高,易过滤,滤液清澈.Validamycin producing strain（Streptomyces hygroscopicus var. JinggangsisYen） are screed by natural selection method, and the higher-yield strains with stable genetic performance are obtained. The effects of carbon source and organic nitrogen source on validamycin producing capability are investigated. The results indicated that Validamycin-bacteria producing capacity is higher with the medium of C： N ratio of 1：0.45 - 0.57 and solid content around 12 %, and easy to filter and get clear filtrate.

关 键 词：井冈霉素菌种 自然选育 发酵 碳源 氮源 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] TQ926[农业科学—植物保护]