紫外酶促法检测血浆及尿中D-乳酸盐  

Detection for D-lactate in plasma and urine by enzymatic UV

作　　者：刘梦琼[1] 林永前[1] 陈代钊[1] 

机构地区：[1]海南省人民医院检验科,海南海口570311

出　　处：《中国热带医学》2009年第8期1611-1612,共2页China Tropical Medicine

摘　　要：目的本研究旨在建立了1种检测血浆和尿液中D-乳酸盐的酶学方法并对该法进行了方法学评价。方法用改良的紫外酶促法对血浆及尿中D-乳酸盐检测分析。结果在浓度为0-500μm血浆和尿标准曲线的线性非常好（相关系数，R=0．99）。血浆和尿液批内变异系数（CV）分别为为3．0％和5．8％；血浆和尿液的批间变异系数分别为7．1％和6．8％；血浆和尿液的平均回收率分别为（103．48．2％）和（100．1±7．2）％；结论该方法具有可接受的精度和回收率，准确快速，不需要大型仪器，可适用常规检测。Objective To establish an enzymatic assay for determination of D-lactate in plasma and urine. Methods D-lactate in plasma and and in urine was assayed enzymatically with a improved UV-spectrophotometer. Results Good linearity was observed at the concentration of 0-500μM plasma and urine standard curve （correlation coefficient,R=0.99）. The within-run coefficients of variations（CV）were 3.0% for plasma and 5.8% for urine. The between run CVs were 7.1% for plasma and 6.8% for urine. The mean recovery with standard deviation（S.D.） was 103.4±8.2% for plasma and 100.1%±7.2% for urine. Conclusions The method proved reliable, accurate and fast with acceptable precision and recovery.

关 键 词：D-乳酸盐 紫外酶促法 

分 类 号：R-33[医药卫生]