凋亡相关基因Bcl-2、Bax与舌鳞状细胞癌的相关性研究  

Relative research of apoptosis related gene Bcl-2,Bax in tongue squamous cell carcinoma

作　　者：王开[1] 朱炎[1] 刘玲珍[1] 康非吾[1] 

机构地区：[1]同济大学附属口腔医院口腔颌面外科,上海200072

出　　处：《口腔医学》2009年第7期337-339,347,共4页Stomatology

基　　金：上海市自然科学基金(06ZR14084)

摘　　要：目的探讨舌鳞癌组织Bcl-2和Bax基因蛋白表达与舌鳞状细胞癌发生、发展的关系。方法采用Envision免疫组织化学两步法检测10例舌慢性炎症标本、45例舌鳞状细胞癌标本及20例癌旁舌组织中Bcl-2的表达水平。结果舌鳞癌组织中Bcl-2基因蛋白阳性表达率82.22%,舌鳞癌组织中Bax基因蛋白阳性表达率62.22%,与炎症组织和癌旁组织相比,差异均有显著意义(P<0.05)。舌鳞癌组织中Bcl-2和Bax基因蛋白的表达呈明显的负相关关系(r=-0.324,P<0.01)。结论舌鳞癌组织中Bcl-2和Bax协同作用参与了舌鳞癌的发生发展,有可能成为早期诊断的指标或基因治疗的靶点。Objective To explore the expression of Bcl-2 and Bax in tongue squamous cell carcinoma. Methods Fifty-five cases of tongue tissue with follow-up information were selected, including 45 cases of tongue squamous cell carcinoma, 10 cases of chronic glossitis tissue and 20 cases of tongue tissues adjacent to cancer. Semi-quantitative immunohistochemistry method（Envision） was used to detect the expression of Bcl-2 in these tissues. Results The positive rates of Bcl-2 and Bax in tongue squamous cell carcinoma were 82. 22 % and 62. 22% , repsectively. There was significant difference of the expression of Bcl-2 and Bax among tongue squamous cell carcinoma and tongue tissues adjacent to cancer and chronic glossitis tissue,and the expression of Bcl-2 was negatively correlated with that of Bax in tongue squamous cell carcinoma tissues （ r = - 0. 324, P 〈 0. 01 ）. Conclusions The expression intensity of Bcl-2 and Bax in tongue squamous cell carcinoma was correlated with the degree of differentiation. The study demonstrated that there were correlations among the occurrence and evolution of tongue squamous ceil carcinoma . It will be the target of gene iherapy in tongue squamous cell carcinoma.

关 键 词：BCL-2 BAX 舌鳞状细胞癌 免疫组化 

分 类 号：R780.2[医药卫生—口腔医学]