CK20 mRNA和hTERT mRNA表达与大肠癌患者外周血微转移的临床研究  

Study on CK20 mRNA and hTERT mRNA expression and tumor micrometastasis in peripheral blood in patients with colorectal carcinoma

作　　者：陈萍[1] 王宁菊[1] 刘新兰[1] 

机构地区：[1]宁夏医科大学附属医院肿瘤内科,宁夏银川750004

出　　处：《第四军医大学学报》2009年第15期1417-1421,共5页Journal of the Fourth Military Medical University

基　　金：宁夏回族自治区卫生厅科技重点计划项目(W2004026)

摘　　要：目的:检测大肠癌患者外周血中细胞角蛋白20(CK20)mRNA和人端粒酶逆转录酶(hTERT)mRNA的表达,探讨其能否作为判断大肠癌患者外周血微转移及预后的分子生物学指标.方法:采用RT-PCR技术检测大肠癌32例、肠道良性病变11例和健康体检者20例的外周血中CK20mRNA和hTERT mRNA的定性和半定量表达.以人胃腺癌细胞株SGC-7901作阳性对照.实验数据采用SPSS13.0forWindows软件包统计分析.结果:①CK20mRNA和hTERT mRNA在大肠癌患者外周血中的阳性表达率分别为50.0%和56.2%,相对系数分别为0.89±0.17和0.88±0.17;在肠道良性病变患者外周血中阳性表达率分别为9.1%和0%,相对系数分别为0.11和0;在健康体检者外周血中阳性表达率及相对系数均为0.大肠癌患者与肠道良性病变患者、健康体检者相比,CK20mRNA和hTERTmRNA的表达均有统计学差异(P<0.05).②CK20mRNA和hTERT mRNA阳性表达率和相对系数与临床分期、远处转移均有关(P<0.05);其阳性表达率与浸润深度、淋巴结转移无关,但相对系数与之均有关(P<0.05).阳性表达者生存率低于阴性者(P<0.05).结论:大肠癌患者外周血中存在CK20mRNA和hTERT mRNA的表达,与临床分期及患者生存密切相关,提示CK20mRNA和hTERTmRNA的表达能预测大肠癌患者外周血微转移,可判断预后.AIM： To detect the expressions of cytokeratin 20 （CK20） mRNA and human telomerase reverse transcriptase （hTERT） mRNA in peripheral blood of patients with colorectal carcinoma and to discuss whether they can be used as molecular biological markers of predicting micrometastasis in peripheral blood and evaluating prognosis. METHODS： Peripheral blood of 32 colorectal carcinoma patients, 11 benign colorectal lesion patients and 20 healthy examination cases was detected by RT-PCR technique for qualitative and semiquantitative analysis of CK20 mRNA and hTERT mRNA. Human SGC-7901 gastric adecell lines were used as positive controls. The results were analyzed by SPSS13.0 software. RESULTS： The positive expression rate of CK20 mRNA and hTERT mRNA in peripheral blood of patients with colorectal carcinoma was 50. 0% and 56.2% （relative coefficient： 0.89 ± 0. 17 and 0.88 ± 0. 17）, and 9.1% and 0% in benign colorectal lesion, （relative coefficient： 0.11 and 0）. CK20 mRNA and hTERT mRNA were not detected in peripheral blood of healthy subjects. Significant differences were found between colorectal carcinoma patients, benign colorectal lesion patients and healthy subjects （ P 〈 0.05, all）. The positive expression rates and relative coefficients of CK20 mRNA and hTERT mRNA were significantly correlated with clinical stage and distant metastasis （ P 〈 0.05 ）. The positive expression rates of CK20 mRNA and hTERT mRNA were not correlated with depth of tumor invasion and lymph node metastasis, while the relative coefficients were correlated with them（ P 〈0.05）. Patients with positive expression had lower survival rates compared with negative expression patients （ P 〈 0.05 ）. CONCLUSION： CK20 mRNA and hTERT mRNA are positively expressed in peripheral blood of patients with colorectal carcinoma. The two markers can be used to predict the micrometastasis in peripheral blood and to the evaluate prognosis in colorectal carcinoma patients.

关 键 词：结直肠肿瘤 外周血 微转移 逆转录聚合酶链反应 CK20 MRNA HTERT MRNA 

分 类 号：R735.3[医药卫生—肿瘤]