Evaluation of PLGA/Chitosan/HA Conduits for Nerve Tissue Reconstruction  

作　　者：申雄军 阮建明 

机构地区：[1]State Key Laboratory of Powder Metallurgy,Central South University [2]College of Chemical and Biological Engineering,Changsha University of Science and Technology

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2009年第4期566-570,共5页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the National Natural Science Foundation of China(No.50774096)

摘　　要：A micro-envioment for nerve cells and tissue growth were designed and constructed via surface modification of poly（L-lactide-co-glycolide）（PLGA） with chitosan and hydroxyapatire（HA）. The poly（L_lactide-co-glycolide）/chitosan/hydroxyapatite （PLGA/chitosan/HA） conduits were manufactured by a combined solvent casting and particulate leaching technique. The conduits were highly porous with an interconnected pore structure and 76.5% porosity. Micropores with 50-100 micrometer diameter were formed in the conduits. In vivo application of PLGA/chitosan/HA conduits for reconstruction of 10 mm sciatic nerve defect was assessed by the walking track analysis, the quantifying of the wet weight of tibialis anterior muscle and the histological assessment. The conduits in host rats in vivo can not only be an effective in promoting regenerating of nerves but can also lead to favorable nerve functional recovery.A micro-envioment for nerve cells and tissue growth were designed and constructed via surface modification of poly（L-lactide-co-glycolide）（PLGA） with chitosan and hydroxyapatire（HA）. The poly（L_lactide-co-glycolide）/chitosan/hydroxyapatite （PLGA/chitosan/HA） conduits were manufactured by a combined solvent casting and particulate leaching technique. The conduits were highly porous with an interconnected pore structure and 76.5% porosity. Micropores with 50-100 micrometer diameter were formed in the conduits. In vivo application of PLGA/chitosan/HA conduits for reconstruction of 10 mm sciatic nerve defect was assessed by the walking track analysis, the quantifying of the wet weight of tibialis anterior muscle and the histological assessment. The conduits in host rats in vivo can not only be an effective in promoting regenerating of nerves but can also lead to favorable nerve functional recovery.

关 键 词：poly（L-lactide-co.glycolide）/chitosan/hydroxyapatite（PLGA/chitosan/ttA） CONDUIT biocompatibility nerve regeneration tissue engineering 

分 类 号：TS106.67[轻工技术与工程—纺织工程]