蚕丝木瓜蛋白酶脱胶工艺条件探讨  

Investigating the Process Conditions of Mulberry Silk Degumming with Papain

作　　者：邓一民[1] 张高军[1] 敬凌霄[1] 温少美[1] 王平[1] 

机构地区：[1]西南大学纺织服装学院,重庆400715

出　　处：《丝绸》2009年第8期20-22,28,共4页Journal of Silk

基　　金：重庆市教委科技项目(KG07218)

摘　　要：研究脱胶条件对蚕丝木瓜蛋白酶脱胶效果的影响。由单因子实验选出合适的酶质量浓度、温度、时间、pH值范围,用正交实验优化脱胶条件。结果表明,木瓜蛋白酶能有效地脱除丝胶;脱胶率和丝的强力损失率,随着酶质量浓度增加、pH值及温度的升高和脱胶时间的延长而上升;正交实验证实酶质量浓度是影响脱胶率的主要因子,酶脱胶时间是强力损失的主要影响因素;综合考虑脱胶率和强力损失率,确定生丝木瓜蛋白酶脱胶的2个较好工艺条件为:一是酶质量浓度3 g/L、pH6、温度85℃、时间60 min;二是酶质量浓度3 g/L、pH8、温度65℃、时间90 min。This study investigated whether the degumming conditions had effect on mulberry silk degumming with papain. It used single-factor experiment to pick up the proper enzyme quality-concentration, temperature, time, pH value range, and then obtained optimized degumming conditions by orthogonal experiment. The results indicated that papain can effectively remove sericin. The results of the single-factor experiments showed the degumming loss rates and silk degumming strength loss rate are increasing with the enzyme concentration increase, pH value and temperature enhance and degumming time extension. The orthogonal experiment confirmed that the enzyme concentration is the main factor impacting on the degumming loss rate, the time of degumming is the major factor of affecting the force loses. Considered degumming loss rate and breaking strength loss rate, two better craft conditions of mulberry silk degumming are： one is papain quality concentration： 3 g/L, pH：6, temperature：85 ℃, time：60 min; the other is papain quality concentration：3 g/L, pH：8, temperature：65 ℃, time：90 min.

关 键 词：木瓜蛋白酶 蚕丝 脱胶率 强力损失率 脱胶条件 

分 类 号：TS192.552[轻工技术与工程—纺织化学与染整工程]