消痰散结方对裸鼠人胃癌MKN-45端粒酶活性表达的影响  

Influence of Xiaotan Sanjie Fang on the expression of human gastric cancer MKN-45 telomerase activity in a nude mice model

作　　者：李春杰[1] 魏品康[2] 余柏林 

机构地区：[1]解放军第211医院干部病房,哈尔滨150080 [2]上海长征医院 [3]黑龙江省哈尔滨市骨伤医院

出　　处：《中国临床保健杂志》2009年第4期391-393,共3页Chinese Journal of Clinical Healthcare

基　　金：中国博士后基金资助项目(20060400639)

摘　　要：目的通过观察消痰散结方对裸鼠人胃癌MKN-45原位移植模型端粒酶活性表达的影响,探讨其抑制胃癌细胞增殖的作用机制。方法采用OB胶粘贴法建立裸鼠人胃癌MKN-45原位移植瘤模型,随机分成模型组、消痰散结方低、中、高剂量组、5-氟脲嘧啶(5-Fu),每组8只,运用PCR-ELISA方法检测端粒酶活性的表达。结果①消痰散结方低、中、高剂量组的平均瘤重与模型组相比,差异有统计学意义(P<0.01);②消痰散结方中剂量组和5-Fu组端粒酶活性与模型组组比较明显降低,差异有统计学意义,P<0.05,消痰散结高剂量组端粒酶活性与模型组组比较差异有统计学意义(P<0.01)。结论消痰散结方抑制胃癌细胞生长,部分可能与其抑制端粒酶活性有关。Objective To discover the mechanism of Xiaotan Sanjie Fang （XTF）, a traditional Chinese medicinal empirical Prescription for treating gastric cancer by observing its influence on the expression of human gastric cancer MKN-45 telomerase activity in a nude mice in situ tumor transplantation model. Methods Using OB glue paste method,the nude mice human gastric cancer MKN-45in situ transplantation tumor model was established. Animals were randomly divided into 5 groups （8 mice/every group） ： the blank model group, low-dose XTF group, median-dose XTF group,high-dose XTF group, and 5-Fu group By PCR-ELISA the expression of telomerase activity was detected. Results ① There was a significant difference in mean tumor weight in the low, median and high dose XTF groups compared with the model group （P 〈 0.01 ） ② Telomerase activity in mid dose group and 5-Fu group was lower than that of normal NS group （ P 〈0. 05 ）, Telomerase activity high dose group was lower than normal NS group （ P 〈 0. 01 ）. Conclusion Xiaotan Sanjie Fang can inhibit the growth of gastric cancer cells, which may be related partly to the inhibition of telomerase activity.

关 键 词：胃肿瘤 端粒 末端转移酶 胆南星 半夏 小鼠 裸 

分 类 号：R735.2[医药卫生—肿瘤]