邻苯二甲酸二(2-乙基)己酯对斜生栅藻的生态毒性作用  

Ecological Toxicity of Di(-2-Ethylhexyl) Phthalate on Scenedesmus Obliquus

作　　者：王莉[1] 唐丽雅[2] 魏晨曦[1] 刘丹丹[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院环境科学实验室与湖北省遗传调控和整合生物学重点实验室,武汉430079 [2]香港理工大学应用生物与化学科技系

出　　处：《生态毒理学报》2009年第3期452-456,共5页Asian Journal of Ecotoxicology

基　　金：国家科技支撑计划项目(No.2006BAJ02A10;No.2006BAI19B05)

摘　　要：为了研究邻苯二甲酸二(2-乙基)己酯(DEHP)对水生藻类的生态毒性作用,以斜生栅藻(Scenedesmus obliquus)为受试对象,设置5个DEHP浓度梯度(5、10、20、40、80mg·L-1)和1个对照组,实验周期为96h,以藻细胞数量和光合色素含量为测试指标,检测了DEHP对斜生栅藻生长的影响.结果表明,DEHP对斜生栅藻生长和色素含量具有一定的影响:暴露96h后,各DEHP暴露组斜生栅藻生物量(藻细胞数)与对照组均有显著差异(p<0.05),且随DEHP暴露浓度的升高,生物量逐渐降低;各DEHP暴露组叶绿素a、b含量均显著低于对照(p<0.05),在较低DEHP浓度(5、10mg·L-1)下,叶绿素a、b含量略呈上升趋势,较高DEHP浓度(≥40mg·L-1)下,则呈下降趋势;DEHP暴露对类胡萝卜素含量影响不大,除80mg·L-1组显著降低(p<0.05)外各实验组差异不大(p>0.05).In order to explore the ecological toxicity of di-（2-ethylhexyl） phthalate（DEHP）on algae, effects of DEHP on the growth of（Scenedesmus obliquus）were studied. The exposure concentrations of DEHP were 0, 5, 10, 20, 40, 80mg·L^-1, After 96h exposure, the cell density and photosynthetic pigment content of Scenedesmus Obliquus were examined. Results showed that, after 96h exposure, the cell densities in exposure groups were significantly lower than those in control group （p〈0.05）. The cell densities decreased with the increase of DEHP concentrations. The contents of Chlorophyll a and Chlorophyll b in exposure groups were also significantly lower than those in control group （p〈0.05）. The contents of Chlorophyll increased slightly at the low concentrations（5, 10mg·L^-1）and decreased at the high concentrations （≥40mg·L^-1）. DEHP had little effect on carotenoid, and no significant difference was found between groups except for the significant decrease of carotenoid content in 80mg·L^-1 group.

关 键 词：邻苯二甲酸二(2-乙基)己酯 斜生栅藻 生态毒性 叶绿素A 叶绿素B 类胡萝卜素 

分 类 号：X171.5[环境科学与工程—环境科学]