中国南海短指软珊瑚中化学成分的研究  

Studies on the Chemical Constituents of the Soft Coral Sinularia sp. from the South China Sea

作　　者：王长云[1] 刘海燕[1] 孙雪萍[1] 李秀保[2] 孙玲玲[1] 刘新[1] 邵长伦[1] 管华诗[1] 

机构地区：[1]中国海洋大学医药学院,海洋药物教育部重点实验室,山东青岛266003 [2]中国科学院南海海洋研究所,广东广州510301

出　　处：《中国海洋大学学报（自然科学版）》2009年第4期735-738,共4页Periodical of Ocean University of China

基　　金：国家908专项项目(908-01-ST12908-02-05-04);国家自然科学基金(40776073);国家科技基础性工作专项项目(2007FY210500);国家教育部科技创新工程重大项目培育资金项目(706038);国家教育部新世纪优秀人才支持计划(NCET-05-0600)资助

摘　　要：运用硅胶柱层析、Sephadex LH-20凝胶柱层析和HPLC等技术,采用活性追踪分离的方法,对南海短指软珊瑚Sinulariasp.的化学成分进行了研究。从乙醇提取物中分离获得单体化合物,运用NMR,MS等波谱技术鉴定了8个化合物的结构,分别为:3β-羟基-24-亚甲基胆甾-5-烯-7-酮(1),孕甾烯醇酮(2),cembrene A(3),epoxycembrane A(4),4,8,12-trimethyl-1-(1-methylethenyl)-3,7-cyclotetradecadien-10-one(5),(3E,7E,11E)-11,12-dihydroxy-1-isopropyl-4,8,12-trimethy-icyclotetradeca-1,3,7-triene(6),邻苯二甲酸二丁酯(7)和十九烷-2-酮(8)。化合物5和8为首次从珊瑚动物中分离得到,6为首次从短指软珊瑚属中分离得到。化合物3和4显示较强的卤虫致死活性。西松烷二萜类化合物是该短指软珊瑚中的重要活性成分。The chemical constituents of soft coral Sinularia sp. from the South China Sea were investigated by bioassay-guided fractionation. The ethanolic extract was separated by repeated column chromatography on silica gel and Sephadex LH-20 and HPLC to obtain eight compounds. Their structures were elucidated as 3β hydroxy-24-methylenecholest-5-en-7-one（1）, pregnenolone（2）, cembrene A（3）, epoxycembrane A（4）, 4, 8, 12-trimethyl-1- （1-methylethenyl）-3, 7-cyclotetradecadien-10-one （5）, （3E, 7E, 11E）-11, 12-dihydroxy-1-iso- propyl-4, 8, 12-trimethyicyclotetradeca-1, 3, 7-triene（6）, 1, 2-benzenedicarboxylic acid, isobutyl ester（7） and 2-nonadecanone（8）, respectively, on the basis of their spectroscopic data including NMR and MS. Compounds 5 and 8 were isolated from corals for the first time, and compound 6 was firstly isolated from the genus of Sinularia. Compounds 3 and 4 exhibited high lethal rates to brine shrimp Artemia salina. Cembrane-type diterpenoids were deduced as important bioactive compounds in the soft coral Sinularia sp.

关 键 词：短指软珊瑚 化学成分 分离 结构鉴定 

分 类 号：P714.5[天文地球—海洋科学] R914[医药卫生—药物化学]