固定化分枝杆菌BD696-6产雄烯二酮发酵条件的研究  

Fermentation of Immobilized Mycobacterium sp.BD 696-6 for Production of Androst-4-ene-3,17-dione from Rapeseed Sterols

作　　者：冯丽桦[1] 潘丽军[1] 姜绍通[1] 杨英[1,2] 罗炳华[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009 [2]安徽建筑工业学院环境工程系,安徽合肥230022

出　　处：《食品科学》2009年第15期172-175,共4页Food Science

基　　金："十一五"国家科技支撑计划项目(2006BAD05A12);安徽省自然科学基金项目(070413265X)

摘　　要：利用海藻酸钙凝胶颗粒将分枝杆菌BD696-6固定,进行固定化细胞的发酵条件研究。实验结果表明:发酵最适条件为:pH8.5、温度32℃、底物浓度0.5%、转化时间144h,较游离细胞发酵周期缩短了24h,雄烯二酮产量明显提高,得率均在78%以上,且固定化细胞的贮藏稳定性、重复使用效果较好。电镜扫描结果显示以海藻酸钙为包埋载体时,营养物质可自由传输。Mycobacterium sp.BD 696-6, a mutant strain of Mycobacterium sp.BD 696-6 that can effectively convert sterols into androst-4-ene-3,17-dione （AD）, was immobilized by entrapment in calcium alginate gel capsules. AD was produced from rapeseed sterols by the fermentation of immobilized Mycobucterium sp.BD 696-6. The effects of initial pH of fermentation medium, temperature, substrate concentration and fermentation time on AD yield were investigated by sing-factor method. In addition, the storage stability and repeated usability of immobilized Mycobacterium sp.BD 696-6 were evaluated and gel capsules embedding Mycobacterium sp.BD 696-6 cells after fermentation were observed under scanning electron microscopy. An AD yield of more than 78% was obtained by the fermentation under substrate concentration 0.5%, initial pH 8.5 and temperature 32 ℃ for 144 h which was shortened by 24 h when compared with the fermentation time of free cell. Immobilized Mycobacterium sp. BD696-6 showed excellent storage stability and after used repeatedly 9 times its mechanical strength and AD conversion capacity had no marked decrease. SEM images revealed that the nutrients for calcium alginate-immobilized Mycobacterium sp. BD 696-6 could be freely transported.

关 键 词：细胞固定化 雄烯二酮 甾醇 海藻酸钙 分枝杆菌 

分 类 号：Q949.3[生物学—植物学]