内源性神经营养因子-3在电针刺激治疗脊髓损伤中的作用  

Effect of endogenous NT-3 on spinal cord injury treated with electro-acupuncture

作　　者：刘芬[1] 张军明[1] 张禅[1] 王旭阳[2] 廖敏[1] 任艳华[1] 屠文展[3] 雷亚宁[1] 

机构地区：[1]浙江温州温州医学院组织胚胎学教研室,325035 [2]温州医学院附属第一医院神经外科 [3]温州医学院附属第二医院康复中心

出　　处：《解放军医学杂志》2009年第8期1000-1001,1008,共3页Medical Journal of Chinese People's Liberation Army

基　　金：浙江省教育厅资助项目(20051156);温州市科技局科研基金资助项目(Y20070056)

摘　　要：目的探讨内源性的神经营养因子-3(NT-3)在成年猫脊髓去传入损伤后电针刺激治疗中的作用。方法成年猫25只,雌雄不拘,随机分为左侧L1-L5、L7-S2背根去传入手术组(手术组,保留L6为备用背根)、手术+电针刺组(电针组)、手术+电针刺+NT-3抗体封闭组(抗体封闭组),2个月后对相应脊髓节段和备用背根节进行霍乱毒素B亚单位结合辣根过氧化物酶复合物(CB-HRP)形态学示踪及免疫组化、酶组化观察并进行组间比较。结果各组背根节感觉神经元中枢投射轴突终末集中分布在脊髓后角Ⅲ、Ⅳ、Ⅴ板层内侧,且电针组感觉神经元突起标记点密度(171.5±12.1/100μm2)明显高于手术组(101.2±6.5/100μm2)及抗体封闭组(142.3±12.3/100μm2,P<0.05)。结论电针刺可能通过上调NT-3的表达促进损伤脊髓和感觉神经元的形态和功能修复而参与脊髓可塑性过程。Objective To explore the effect of endogenous neurotrophin-3 （NT-3） on dorsal rhizotomy spinal cord injury in adult cats treated by electro-acupuncture （EA）. Methods Dorsal rhizotomy at L1-L5 and L7-S2 was performed in 25 cats with preservation of L6 dorsal mot ganglion as standby, the animals were then randomly divided into three groups： operation group （n=10） ; EA group （n=10）, animals were treated with EA after operation; and blocking group （n=5）, animals were treated with EA and NT 3 blocking antibody after operation. Two months after dorsal rhizotomy, the spinal cord and dorsal root ganglion （DRG） of L6 segment were observed using CB- HRP retrograde tracing methods, immunohistochemistry and enzymohistochemistry analysis, and the results were compared among groups. Results With CBHRP tracing, it was found that the regeneration sprouting of large sensory neuron of DRG were distributed mainly in the internal side of Ⅲ, Ⅳ and Ⅴ laminas of spinal dorsal horn; the density of terminal sprouting derived from spared DRG were greatly increased in EA group （171.5± 12. 1/100μm^2 ）, and significantly higher than that of operation group （101.2 ± 6. 5/100μm^2 ） and blocking group （142.3± 12. 3/100μm^2, P〈0. 05）. Conclusion EA may take part in the spinal cord remodeling by up-regulating the expression of NT-3, which may promote the repair of morphology and function of injured spinal cord and sensory neurons in DRG.

关 键 词：神经营养因子3 电针 神经元可塑性 神经元 传入 

分 类 号：R651.22[医药卫生—外科学] Q813.11[医药卫生—临床医学]