牛泰勒虫18S rRNA基因序列比较研究  

Studies on Comparison of 18S rRNA Gene Sequences of Theileria Species Infective to Cattle

作　　者：刘爱红[1] 关贵全[1] 刘军龙[1] 李有全[1] 马米玲[1] 牛庆丽[1] 任巧云[1] 殷宏[1] 罗建勋[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,农业部草食动物疫病重点实验室,兰州730046

出　　处：《畜牧兽医学报》2009年第7期1063-1068,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然资源平台项目(2005DKA21100);国家自然科学基金项目(30571397);国家“863”项目(2006AA10A207);科技部欧盟项目专项经费;中国农业科学院杰出人才基金;欧盟EPIZONE、ICTTD3(INCONo.510561SSA-INCOME项目

摘　　要：作者对中国兽医微生物菌种保藏中心原虫资源库内收藏的3种牛源泰勒虫10个地方分离株（包括4株环形泰勒虫、3株瑟氏泰勒虫和3株中华泰勒虫）的18S rRNA基因序列进行了测定，并用所测得的牛的泰勒虫中国株基因序列和自GenBank下载的各种泰勒虫18S rRNA基因序列构建了系统发生树。结果显示：牛的3种泰勒虫18S rRNA基因大小在1740-1890 bp，并有规律地分布于3个不同的进化枝上，且种内地方分离株之间的同源性明显大于种间同源性，说明采用18S rRNA基因序列测定方法对牛的泰勒虫进行分类与传统分类方法具有较好的符合性。该研究也可为建立针对18S rRNA基因为靶标的牛的泰勒虫病PCR检测方法奠定基础。Ribosomal small-subunit RNA gene sequence of ten Chinese Theileria strains infective to cattle, including four isolates of Theileria annulata, three isolates of T. sergenti and three isolates of T. sinensis, collected in the protozoon resource library at the center-conservation of strain in China veterinary microbion, were cloned and analyzed and then the phylogenetic trees were constructed based on 18S rRNA sequence of the Chinese isolates as well as other species of Theileria available in GenBank. The result indicated that the length of the 18S rRNA gene of all Theileria species involved in this study were varied between 1 740--1 890 bp and Chinese isolates of three Theileria isolates were located in the different phylogenetic branch, moreover, the percent identities of the different isolates in species were much higher than among species. The results were basically consistent with traditional classification as well as molecular classification based on 18S rRNA gene sequence for the bovine Theileria. This study will provide a foundation for PCR detected method for bovine theileriosis.

关 键 词：牛 泰勒虫 18S RRNA基因 系统发生树 分类 

分 类 号：S852.72[农业科学—基础兽医学]