肉牛PRKAG3基因多态性及其与胴体和肉质性状的关联分析  

Polymorphism of PRKAG3 Gene and Its Association with Carcass and Meat Quality Traits in Beef Cattle

作　　者：李武峰[1] 杨润军[2] 甘乾福[3] 张路培[3] 许尚忠[3] 李俊雅[3] 高雪[3] 

机构地区：[1]山西农业大学动物科技学院,太谷030801 [2]西北农林科技大学动物科技学院,杨凌712100 [3]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《畜牧兽医学报》2009年第7期1106-1111,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家“十一五”科技支撑计划课题(2006BAD01A10);“十一五”国家高技术863计划课题(2008AA10Z146);山西农业大学科技创新基金(200201)资助

摘　　要：本研究以晋南牛、鲁西牛、西门塔尔、安格斯、海福特、利木赞和夏洛莱7个品种共267头肉牛为试验材料,采用PCR-RFLP技术检测PRKAG3基因多态性,找到了PRKAG3基因DNA序列中的2个突变位点T2643C和T2885C(DQ082736),同属第4内含子。2个基因多态性位点与肉牛胴体和肉质共9个性状的关联分析表明:PRKAG3基因T2885C变异位点的3个基因型间嫩度性状差异显著(P<0.05),CC基因型最小二乘均值显著低于CD和DD基因型最小二乘均值。本研究结果可为肉牛肉质性状标记辅助选择提供依据。Polymorphisms of PRKAG3 gene were detected in 267 beef cattle in 7 beef cattle breeds （Simmental, Angus, Hereford, Charolais, Luxi cattle, Limousin and Jinnan cattle） by PCRRFLP. Two polymorphic sites T2643C and T2885C were found（DQ082736）, both of them in intron 4. Association analysis of the two SNPs with 9 carcass and meat quality traits was carried out. The results showed that T2885C substitution of PRKAG3 gene is significantly associated（P 〈0.05） with meat tenderness, and the mean value of individuals with genotype CC is lower than that of individuals with genotype CD and DD. The information found in the present research is very important for meat quality breeding in beef cattle by marker-assisted selection.

关 键 词：肉牛 PRKAG3基因 胴体性状 肉质性状 关联分析 PCR-RFLP 

分 类 号：S823.92[农业科学—畜牧学] Q343.15[农业科学—畜牧兽医]