钌多吡啶配合物与DNA相互作用的发光动力学分析  

Luminescence Dynamics Analysis on Ruthenium Polypyridyl Complexes Bonding to DNA

作　　者：朱伟玲[1,2] 刘学文[3,4] 王惠[2] 郑康成[4] 计亮年[2,4] 

机构地区：[1]茂名学院物理系,广东茂名525000 [2]中山大学光电材料与技术国家重点实验室,广东广州510275 [3]湖南文理学院化学化工系,湖南常德415000 [4]中山大学化学与化学工程学院生物无机与合成化学教育部重点实验室,广东广州510275

出　　处：《光谱学与光谱分析》2009年第8期2050-2053,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(60478013;20571089);国家重点基础研究发展计划"973"项目(NO2007CB815306);茂名学院科研基金项目(203346)资助

摘　　要：采用三能级模型,对六种钌多吡啶配合物[Ru(L)2(R)]2+(L=bpy,phen,bpy=2,2’-联吡啶,phen=1,10-邻菲咯啉,R=7-CH3-dppz,7-F-dppz,dpbpd(NH2)2)水溶液与小牛胸腺DNA相互作用的时间分辩发光光谱进行对比分析,讨论了取代基对配合物与DNA作用时方式的影响。结果表明:(1)六种配合物与DNA作用存在侧面插入方式和垂直插入方式,其中垂直插入方式的权重较大;(2)取代基的性质对两种作用方式的权重有重要影响。上述结论为进一步研究配合物分子与DNA的相互作用的机理提供了动力学依据。Time-resolved spectra of six kinds of ruthenium polypyridyl complexes [Ru（L）2 （R）]^2＋ （L= bpy, phen, bpy= 2,2＇- bipyridine, phen=1,10-phenanthroline, R= 7-CH3-dppz, 7-F-dppz, dpbpd（NH2 ）2 ） bonding to calf thymus DNA in aqueous solution were compared and analyzed by using the three energy level kinetic model. And the effects of the substituent groups on the interaction ways for the ruthenium complexes bonding to DNA were discussed. The result shows that first, the six complexes all show two binding modes on bonding to DNA, i.e. the side-on binding mode and the perpendicular binding mode, and the later one is considered as a main binding way. Second, the properties of substituent groups have an important impact on the relative weight of the two binding modes. The conclusion offers a dynamics argument to study the interaction mechanism for the complex bonding to DNA further.

关 键 词：超快激光与光电子学 瞬态发光 时间分辩光谱技术 钌配合物 DNA 

分 类 号：O482.31[理学—固体物理]