非正常形态红细胞对拉曼光谱判别的影响  

The Effect of Abnormal Cell Shape on the Spectral Distinguishing of Erythrocytes Using Laser Tweezers Raman Spectroscopy

作　　者：王桂文[1] 彭立新[1] 姚辉璐 黄庶识[1] 陈萍[2] 黎永青[3] 

机构地区：[1]广西科学院生物物理实验室,广西南宁530003 [2]广西医科大学,广西南宁530021 [3]美国东卡罗莱纳大学物理系

出　　处：《光谱学与光谱分析》2009年第8期2117-2121,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金资助项目(30660063);广西科学院科技创新项目(桂科院研0702)资助

摘　　要：血红细胞对环境敏感,细胞形态很容易发生改变,皱缩甚至形成棘形。文章应用光镊拉曼光谱技术俘获、收集来自健康个体和地中海贫血患者的正常形态、一般皱缩和棘形红细胞的拉曼光谱,从平均光谱、主成分分析等方法分析不同形态红细胞的光谱差异与胞内Hb的变化,及其对光谱诊断分析的影响。结果发现,在正常的生理环境下,红细胞发生皱缩甚至形成棘形细胞并不影响光谱判别分析。Erythrocyte is a mature blood cell that contains hemoglobin to carry oxygen to the bodily tissues. Erythrocyte, which takes on a biconcave disc that has no nucleus, is flexible and changeful. Erythrocyte is so sensitive to the environment that the shape of cell goes crimpy, even acanthoid. A laser tweezers Raman spectroscopy （LTRS） setup was used to trap single erythrocyte from healthy donors and patients with thalassemia and to collect the Raman scattering of trapping cell. Normal shape, crimpy erythrocytes and acanthoid erythrocytes were tested, and the averaged spectra, and principal component analysis （PCA） which detailed the spectral difference and the change of hemoglobin, were used to evaluate the effects of different cell shape on the spectral distinguishing of erythrocyte. The results reveal that in normal physiological environment the change in cell shape does not effect the spectral distinguishing of abnormal erythrocyte.

关 键 词：地中海贫血 皱缩红细胞 棘形红细胞 拉曼光谱 

分 类 号：O657.3[理学—分析化学]