结直肠癌肝转移组织中胸苷磷酸化酶表达  

Expression of thymidine phosphorylase in human metastatic liver cancer tissues from colorectal cancer

作　　者：游伟[1] 张继民[1] 邹湘才[1] 郝卓芳[2] 廖德贵[2] 黄世章[2] 洪楚原[1] 

机构地区：[1]广州医学院第二附属医院胃肠外科,广州510260 [2]广州医学院第二附属医院病理科,广州510260

出　　处：《国际外科学杂志》2009年第7期440-444,505,共6页International Journal of Surgery

基　　金：国家自然科学基金资助项目（No.30471998）;广东省自然科学基金资助项目（No.04009573）;教育部留学回国人员科研启动基金（No.教外司留[2002]247号）

摘　　要：目的检测胸苷磷酸化酶（TP）在人结直肠癌肝转移组织中的表达，分析该表达与肿瘤相关巨噬细胞（TAM）及与患者预后的相关性。方法采用抗TP的单克隆抗体654—1及1C6-203、抗巨噬细胞标记物CD68的单克隆抗体PG—M1，对28例人结直肠癌肝转移标本进行免疫组织化学染色分析，对3种抗体阳性细胞进行细胞计数，计算各种阳性细胞之间，以及各种阳性细胞与患者预后的相关性。结果28例标本中，转移癌细胞表达TP阳性的仅2例，癌组织中表达TP的阳性细胞主要是癌巢周围的间质细胞，特别是肿瘤相关巨噬细胞（TAM）。通过对3种阳性细胞计数发现，2种TP抗体染色阳性细胞数相关（r=0．697，P〈0．01）；654—1阳性细胞数与TAM细胞数相关（r=0．703，P〈0．01）；而1C6-203阳性细胞数与TAM细胞数相关较弱，无统计学意义（r=0．359，P=0．06）。16例死亡患者的术后生存时间与3种阳性细胞数目均无相关（P〉0．05）。结论在人结直肠癌肝转移组织中表达TP阳性的细胞主要是TAM，组织中TP阳性细胞及CD68阳性巨噬细胞的多少与患者预后生存的时间无相关。Objective To detect the thymidine phosphorylase （TP） expression in metastatic liver cancer tissues from human eolorectal cancer by immunohistoehemistry, and analyze the correlation between TP expression and the tumor-associated maerophages （TAM）, and the prognosis of patients. Methods Twentyeight metastatic liver cancer specimens resected from patients with eoloroetal cancer, were immunohistochemieally stained by 654-1, an anti-TP monoelonal antibody, 1C6-203, another anti-TP monoclonal antibody, PG-M1, anti-maerophage marker CD68 monoelonal antibody. Morphometrical analysis and positive cell counting were performed, and the correlation of TP expression with the patient＇s prognosis was evaluated. Results In normal liver tissues, the hepatic ceils apart from cancer nests were weakly positive for 654-1 as well as for 1 C6-203. The most TP-positive cells were distributed mainly along the invasive margin of cancer or around the cancer nests. In the corresponding areas, CD68-positive maerophages were also increased. The distribution patterns of CD68-positive cells were similar to those of TP-positive cells. The numbers of the TP- positive cells stained by 654-1 were significantly correlated with numbers of 1C6-203 positive cells （r = 0.697, P 〈 0.01 ）, also correlated with the numbers of CD68-positive cells （ r = 0.703, P 〈 0.01 ）. While the numbers of 1C6-203 positive cells had no significant differences with the numbers of CD68-positive cells （ r = 0.359, P 〉 0.05 ）. The TP-positive cancer cells both for 654-1 and for 1 C6-203 were detected only in 2 of 28 specimens. Both the number of TP-positive cells for 654-1 and 1 C6-203, and the number of CD68- positive cells had no correlation with the survival period of patients. Conclusions In the metastatic liver cancer tissues of human colorectal cancer, the TP-expression stained by 654-1 was coincidence with 1 C6- 203, and the most important source of TP-expression is the TAM in stromal tissues around cancer nests, while the cancer cells are l

关 键 词：胸苷磷酸化酶 结直肠癌 肝转移 肿瘤相关巨噬细胞 预后 

分 类 号：R735.3[医药卫生—肿瘤]