神经营养因子NT-3神经干细胞移植对帕金森病大鼠功能修复研究  

Effects of Neurotrophic Factor NT-3 and Neural Stem Cell on the Function Repair for Parkinson Disease Model

作　　者：毕建清[1] 姚远[1] 文铁桥[1,2] 

机构地区：[1]上海大学生命科学学院,上海200444 [2]上海大学系统生物技术研究所,上海200444

出　　处：《科技导报》2009年第14期48-53,共6页Science & Technology Review

基　　金：国家重点基础研究发展计划(973计划)项目(2006cb500700)

摘　　要：为了研究神经干细胞和神经营养因子NT-3对帕金森病(PD)的修复作用,将NT-3表达载体pEGFP-C1-NT3转染大鼠原代神经干细胞(NSCs),构建了NSCs-NT3细胞。移植入帕金森病模型大鼠脑部MFB和VTA区域,观察到移植细胞在大鼠移植部位生长与迁移,证实移植成功。对移植后疾病模型进行APO旋转实验和水迷宫行为检测,结果表明,NT-3有利于学习与记忆能力改善,分子水平证实移植区域NT-3显著表达。本研究通过移植过表达NT-3的神经干细胞,从分子、细胞、组织和个体行为等不同层面证实了NSCs-NT3对PD模型大鼠修复的积极作用,为PD的转基因神经干细胞移植治疗研究提供了理论与实践依据。Neural stem cell transplantation is one of the important ways for repair of nerve injury. In this study, the effects of neural stem cells and neurotrophie factor-3 （NSCs-NT3） on the Parkinson disease model are studied. By transplantation of NSCs-NT3 into lesion regions of PD rats, rotational behavior and Morris Water Maze （MWM） were tested. The results show that the rotation numbers in apomorphineinduced NSCs-NT3 groul5 showed a higher decrease than that of NSCs group. MWM training also suggested NSCs-NT3 improved the spatial learning ability of PD rats. This research demonstrated in the level of molecular, cell, tissue and rats behavior that the overexpression of NT-3 in NSCs-NT3 had positive functions for repair of PD rat models. These results supplied the theoretic and practical foundation for treatment of the genetically-modified NSCs graft.

关 键 词：帕金森病 神经干细胞 神经营养因子-3 移植 

分 类 号：R742.5[医药卫生—神经病学与精神病学]