益气养阴方对子宫颈癌细胞VEGF及MMP2表达的影响  

Effect of Reinforcing Qi and Nourishing Yin on VEGF and MMP2 Expression in Cervical Carcinoma Cells

作　　者：孙静[1] 张丹[1] 朱庆均[1] 李哲[2] 

机构地区：[1]山东中医药大学,250355 [2]山东省济南市中心医院,250013

出　　处：《实用癌症杂志》2009年第3期237-239,共3页The Practical Journal of Cancer

基　　金：山东省中医药管理局课题(2005-115);山东省高等学校优秀青年教师国内访问学者经费资助项目

摘　　要：目的探讨益气养阴方对子宫颈癌细胞血管内皮生长因子(VEGF)和细胞基质金属蛋白酶2(MMP2)表达的影响。方法复制小鼠子宫颈癌U14自发性转移模型,应用免疫组织化学及其组织形态学定量分析方法,检测子宫颈癌中VEGF和MMP2的表达。结果益气养阴方组VEGF表达无强阳性者,表达阳性11例(22%);益气养阴方组MMP-2表达强阳性7例(14%),阳性4例(8%)。益气养阴方组VEGF和MMP2表达水平均明显低于对照组,有显著性差异(P<0.01)。结论益气养阴方对肿瘤血管形成有明显抑制作用,降低VEGF和MMP2的表达可能是其抑制肿瘤血管形成的主要机制之一。Objective To study the role of Reinforcing Qi and Nourishing Yin on vascular endothelial growth factor （VEGF） and matrix metalloproteinase 2 （MMP2） in cervical carcinoma cells. Methods Cervical carcinoma U14 spontaneous metastasis model was adopted. The expression of VEGF and MMP2 in cervical carcinoma was investigated by immunohistochemical and morphological analytics methods. Results Reinforcing Qi and Nourishing Yin group has no strong positive and 11 medium positive （22%） in the expression of VEGF. It has 7 strong positive （ 14% ） and 4 medium positive （8%） in the expression of MMP2. The expression of VEGF and MMP2 in Reinforcing Qi and Nourishing Yin group were lower than the control group（ P 〈 0.01 ）. Conclusion Reinforcing Qi and Nourishing Yin has inhibitory effects on angiogenesis. Its mechanism may be associated with the down-regulating of VEGF and MMP2 expression.

关 键 词：益气养阴方 血管内皮生长因子 基质金属蛋白酶2 血管生成 

分 类 号：R737.33[医药卫生—肿瘤]