IL-21对Jurkat细胞增殖及WAVE-1表达的影响  

Effects of Interleukin-21 on Apoptosis of Jurkat Cells and Expression of WAVE-1

作　　者：吴东红[1] 杨志刚[1] 李庆华[1] 

机构地区：[1]广东医学院附属医院血液内科,广东省湛江市524001

出　　处：《中华全科医学》2009年第8期789-790,共2页Chinese Journal of General Practice

摘　　要：目的探讨白介素-21(IL-21)对Jurkat细胞增殖的抑制作用及WASP家族Verprolin同源蛋白-1(WAVE-1)在急性白血病(AL)发病中可能的作用及意义。方法以不同浓度IL-21处理Jurkat细胞;通过MTT(噻唑兰比色法)检测观察IL-21对Jurkat细胞生长的影响;流式细胞仪观察24h后IL-21对细胞凋亡的影响;采用RT-PCR检测WAVE-1的表达。结果IL-21能明显抑制Jurkat细胞增殖,抑制作用呈剂量时间依赖效应(P<0.05);IL-21作用24h后,细胞凋亡随药物浓度增高而增加(P<0.05);WAVE-1 mRNA表达水平随IL-21处理浓度的增高而降低(P<0.05)。结论IL-21可以抑制人急性白血病细胞的生长和诱导其凋亡,其机制可能与降低WAVE-1的表达有关。Objective To study the effects of Interleukin-21 （IL-21） on apoptosis of Jurkat cells and the probable functions of WAVE-1 in acute leukaemia （AL）. Methods MTT was used to investigate the proliferation of Jurkat cells, flow cytometry （FCM） was used to investigate the effects of IL-21 on apoptosis of Jurkat cells. The expression of WAVE-1 in Jurkat ceils treated with different concentraction of IL-21 was examined by RT-PCR. Results The growth of Jurkat ceils were inhibited by IL-21 in the dose dependent manner（ P 〈 0.05 ）. FCM showed that the apoptosis rates of cells in experimental group were higher than those in control group, with significant difference（P 〈 0.01 or 〈 0.05）. The expression rates of WAVE-1 were decreased by the treatment with the increasing concentrations of IL-21 （P 〈 0.05）. Conclusion IL-21 can restrain the human leukemic cell growth and induce cell apoptosis,which may be related to the expressions of WAVE-1 decreased.

关 键 词：IL-21 JURKAT细胞 凋亡 WAVE-1 

分 类 号：R733.7[医药卫生—肿瘤]