大趾鼠耳蝠线粒体DNA控制区结构及变异  

Structure and Sequence Variation of the Mitochondrial DNA Control Region in Myotis macrodactylus

作　　者：刘丰[1] 宋雨[1] 颜识涵[1] 罗金红[1] 冯江[1] 

机构地区：[1]东北师范大学城市与环境科学学院,长春130024

出　　处：《动物学杂志》2009年第4期19-27,共9页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.30770361);教育部博士点基金项目(No.20060200007);东北师范大学国家大学生创新性实验计划项目(No.071020046)

摘　　要：采用PCR产物直接测序法首次测定大趾鼠耳蝠(Myotis macrodactylus)10个个体的线粒体DNA(mtDNA)控制区全序列,并进行了结构和变异分析。结果表明,大趾鼠耳蝠的控制区结构与其他哺乳动物相似,可分为一个中央保守区(包括F、E、D、C、B元件)和两个外围结构域:延伸的终止结合序列区(包括ETAS1和ETAS2元件)和保守序列区(包括CSB1、CSB2和CSB3元件),其中最为保守的是中央保守区(核苷酸变异度为1.8%)。大趾鼠耳蝠控制区核苷酸全序列具有丰富的长度多态性(1778～2048bp),主要是由在碱基组成、重复数目和排列方式上异质的串联重复序列造成的。在ETAS内发现了TACAT及其反向互补序列ATGTA,支持滑移错配模式(slipped mispairing model)。本研究为该物种的进一步研究和保护提供基础遗传数据。Using PCR product direct sequencing, mitochondrial control region （CR） sequences of 10 individuals of Myotis macrodactylus were analyzed for the first time. The CR sequences of M. macrodactylus, as in other mammals, could be subdivided into a central conserved domain （CD, including five boxes：F, E, D, C and B） and two flanking variable domains： extended termination associated sequences （ETAS, including two conserved elements：ETAS1 and ETAS2） and conserved sequences blocks （CSB,including three conserved elements： CSB1, CSB2 and CSB3）. The CD was the most conserved domain with the nucleotide variance rate of 1.8 % . Because of the repeated sequences which were different in base composition, copy number and arrange type, the CR sequences showed notable length polymorphism （1 778 - 2 048 bp）. In the ETAS, the short sequence TACAT and its reverse complementary sequence were found seven times, supporting the slipped mispairing model. This paper has provided basic genetic data for further study and protection of M. macrodactylus.

关 键 词：大趾鼠耳蝠 控制区 保守元件 串联重复 

分 类 号：Q75[生物学—分子生物学]