石蜡半薄切片制作透射电镜的方法  

Sample preparation methods of TEM using semi-thin paraffin sections

作　　者：曲淑贤[1] 吕广艳[1] 高船舟[1] 魏晓晴[1] 崔颖[1] 高颖[1] 

机构地区：[1]大连医科大学辽宁省医学细胞分子生物学重点实验室,辽宁大连116044

出　　处：《大连医科大学学报》2009年第4期355-356,360,共3页Journal of Dalian Medical University

摘　　要：[目的]介绍石蜡半薄切片转制透射电镜的制样方法。[方法]对石蜡半薄切片进行脱蜡、水化、锇酸固定、缓冲液漂洗、梯度酒精脱水、环氧树脂包埋、超薄切片机切片、透射电镜下观察。[结果]组织细胞的超微结构保存完好,足以满足病理诊断之用。[结论]用石蜡半薄切片转制电镜比用石蜡组织块转制电镜方法简单,快速易行。[ Objective] To introduce sample preparation methods of TEM using semi -thin paraffin sections. [ Methods ]The semi - thin paraffin section was processed with dewaxing, hydration, osmium tetroxide fixed, buffer rinsing, gradient alcohol dehydration, epoxy resin embedded and ultra - thin slice. Then the sections were observed under the Transmission Electron Microscopy. [ Results ] The ultra - structure of histo - cyte remained well. It was sufficient to meet the requirement of pathological diagnosis. [ Conclusion] Compared with paraffin tissue samples, using semi - thin paraffin sections are more efficiency.

关 键 词：石蜡半薄切片 电镜样品制作 透射电镜 

分 类 号：R361[医药卫生—病理学]