高效毛细管电泳法测定大蒜中蒜氨酸含量  

Determination of the content of alliin in Allium Sativum Linn by High Performance Capillary Electrophoresis(HPCE)

作　　者：马晓丽[1] 常军民[2] 孟磊[1] 陈坚[2] 

机构地区：[1]新疆医科大学分析测试中心,新疆乌鲁木齐830011 [2]新疆医科大学药学院,新疆乌鲁木齐830011

出　　处：《新疆医科大学学报》2009年第7期873-874,877,共3页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区高技术研究发展计划(编号:200511112)

摘　　要：目的:建立高效毛细管电泳测定大蒜中蒜氨酸含量的方法。方法:检测条件:熔融石英毛细管50 cm×50μm,未涂层;磷酸氢二钠和磷酸二氢钠缓冲溶液20 mmol/L,pH6.0;分离电压20 kV(+)→(-);电泳时间10 min,电泳温度25℃,压力进样0.5psi×10sec,柱上检测UV 196 nm(二极管阵列检测器)。结果:样品可在8 min内出峰,测得蒜氨酸浓度在80～200μg/ml范围内与峰面积的线性关系良好,相关系数r=0.996 8,平均回收率为97.4%,批内RSD为3.6%。结论:本方法准确、简单、快捷,适用于大蒜中蒜氨酸含量的检测,以控制大蒜药材的质量。Objective： To establish a HPCE method to determine the content of alliin in Alliurn Sativum Linn. Methods： Bare fused-silica capillary 50 cm× 50 /μm; phosphate buffer 20 mmol/L at pH 6.0; running voltage 20 KV; polarity from anode （inlet） to cathode （outlet）; separate time 10 rain, temperature 25℃, sampling 0.5 psi× 10 s, on line detection at 196 nm （PDA）. Results： The sample could be separated in 8 min. The linearity relationship of Alliin concentration and peak value was good in the range of 80-200 μg/ml. The related coefficient was r =0. 996 8, the average recovery of sample for alliin was 97.4%, and the RSD of inter-day was 3.6 %. Conclusion： This methods is simple, quick, accurate and suitable for the quality control for Alliurn Sativurn Linn.

关 键 词：高效毛细管电泳 大蒜 蒜氨酸 含量测定 

分 类 号：R93[医药卫生—生药学] R927.2[医药卫生—药学]