壬基酚对黑斑蛙神经活动的影响  

Effects of nonylphenol on Rana nigromaculata nerve action

作　　者：黄敏毅[1] 俞丽丽[1] 段仁燕[1] 王倩[2] 

机构地区：[1]安庆师范学院生命科学系,安徽安庆246011 [2]陕西师范大学生命科学学院,西安710062

出　　处：《生态学杂志》2009年第8期1510-1514,共5页Chinese Journal of Ecology

基　　金：安徽省教育厅自然科学基金项目(2006kj222B);安徽省高校青年教师科研资助项目(2006jql214)

摘　　要：应用电生理的方法研究不同浓度壬基酚对黑斑蛙的坐骨神经干神经冲动产生和传导的影响。用不同浓度的壬基酚处理黑斑蛙,7d后,观察其活动状态和体表特征,同时用生物信号采集处理系统分别测定壬基酚对黑斑蛙坐骨神经干的神经冲动传导速度、动作电位幅度、相对不应期和绝对不应期的影响。结果表明:随着壬基酚浓度的增加,黑斑蛙的活力减弱,其皮肤出现血斑的现象加重,说明壬基酚可引起黑斑蛙活力、精神和体表等发生异常;随着壬基酚浓度的升高,黑斑蛙坐骨神经干的神经冲动传导速度逐渐减慢,动作电位峰值降低,相对不应期和绝对不应期逐渐延长,与壬基酚浓度呈剂量-效应关系。说明在壬基酚作用下,黑斑蛙神经活动对刺激反应的灵敏性降低,动作电位的产生及传导受到一定程度的抑制和阻碍。在50mg·kg-1低浓度组,壬基酚对黑斑蛙神经活动影响不显著(P>0.05),说明黑斑蛙对低浓度的壬基酚有一定的耐受力。Electrophysiologieal methods were adopted to study the effects of different concentration nonylphenol （NP） on the impulse generation and conduction of sciatic nerve stem in Rana nigromaculata. After treated with different concentration NP for 7 days, the activity and cutis characteristics of R. nigromaculata were examined, and the performance parameters of sciatic nerve stem, including impulse conduction velocity, action＇ s potential peak value, and relative and absolute refractory periods, were measured with biological signal recording and processing system. Increasing NP dosage weakened the R. nigromaculata activity, and produced more blood Spots on cutis, suggesting that NP could induce the abnormality of R. nigromaculata activity, energy, and cutis. When the NP concentration increased, the impulse conduction velocity of sciatic nerve stem gradually slowed down, the peak value of action＇ s potential decreased, and the relative and absolute refractory period extended, which implied that there was a NP dosage-effect relationship. NP could decrease the sensitivity of R. nigrornaculata sciatic nerve, and inhibit its impulse generation and conduction. No significant difference was observed in the nerve action between the control and the treatment 50 mg NP · kg^-1, suggesting that R. nigromaculata had definite tolerance against lower concentration NP.

关 键 词：壬基酚 黑斑蛙 坐骨神经 神经活动 

分 类 号：X174[环境科学与工程—环境科学]