体外快速构建角膜内皮的初步研究  

Preliminary Study of a Rapid Method to Reconstruct Corneal Endothelium in Vitro

作　　者：屈雷[1,2] 敬晓棋[1] 杨学义[2] 闫海龙[1] 窦忠英[2] 

机构地区：[1]榆林学院生命科学研究中心,陕西榆林719000 [2]西北农林科技大学,陕西省于细胞工程技术研究中心,陕西杨凌712100

出　　处：《西北农业学报》2009年第4期33-37,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金项目(39970363);国家重点基础研究发展规划项目(G1999054300)

摘　　要：用培养的角膜内皮细胞在羊膜和去除Descemet膜的角膜基质体外快速构建角膜内皮组织。分离家兔角膜内皮细胞并传代,将2~4代的角膜内皮细胞以1×105~5×105/mL的浓度接种到去上皮的人羊膜和去除Descemet膜的兔角膜基质上,体外快速构建角膜内皮层。角膜内皮细胞在羊膜和去除Descemet膜的角膜基质上培养48 h,细胞就可形成与在体角膜内皮类似的单层角膜内皮组织,并发现角膜基质透明度随着内皮细胞的增殖而逐渐增加的过程。以1×105~5×105/mL的浓度接种到去上皮的人羊膜和去除Descemet膜的兔角膜基质上,48 h可构建与在体角膜内皮类似的单层内皮组织。To establish a rapid method to reconstruct a corneal endothelial tissue in vitro by seeding corneal endothelial cells on amniotic membrane and the corneal stroma removed Descemet membrane. Corneal endothelial cells were separated from rabbit and cultured in vitro. The passage 2 to passage 4 cells with the concentration of 1 × 10^5- 5 × 10^5 cells/mL were seeded on amniotic membrane or the corneal stroma removed Descemet membrane. 48 hours later, a dense monolayer corneal endothelial tissue as in vivo were formed while corneal endothelial cells were seeded on amniotic membrane or the corneal stroma removed Descemet membrane and its thickness was increasing gradually. A dense monolayer corneal endothelial tissue as in vivo can be reconstructed in 48 hours while corneal endothelial cells with the concentration of 1 × 10^5 - 5 × 10^5 cells/mL were seeded on amniotic membrane or the corneal stroma removed Descemet membrane.

关 键 词：组织工程 角膜内皮细胞 体外构建 

分 类 号：R772[医药卫生—眼科]