未分类肾细胞癌的临床病理与染色体畸变研究  

The study on clinical pathology and chromosome aberrations of undifferentiated renal carcinoma

作　　者：康雪玲[1] 邹泓[2] 花本林[1] 尹亮[1] 胡建明[1] 李锋[2] 

机构地区：[1]石河子大学医学院 [2]石河子大学医学院病理学教研室,新疆石河子832002

出　　处：《农垦医学》2009年第3期198-201,共4页Journal of Nongken Medicine

基　　金：国家自然科学基金(30560169);石河子大学高层次人才科研启动基金(RCZX200784)

摘　　要：目的:探讨未分类肾细胞癌(undifferentiated renal carcinoma,URCC)基因组DNA的变化特征。方法:应用比较基因组杂交(CGH)技术分析2例未分类肾癌患者。根据组织学分型、临床分期、性别和年龄进行分组比较。结果:①2例URCC CGH分析结果显示:URCC中发生DNA拷贝数扩增最常见部位是1P和3q,其他依次是2q、16q和1q(>50%);DNA拷贝数缺失最常见的部位是17p,其他依次6p、16p、20p、17q、5p、3p和11q(>50%)。②2例URCC免疫组化表达CD10,CK,VIM,P53强阳性表达。结论:①1P、3q、2q、16q和1q的扩增及2p、6p、16p、20p、17q、5p、3p和11q的缺失可能与URCC发病相关。②17p缺失可能与URCC的高级别类型、预后差相关。Objective： To characterize the profile of chromosomal imbalances of undifferentiated renal carcinoma （URCC）. Methods： Comparative genomic hybridization （CGH） was used to investigate genomic imbalances in 2 cases of URCC, with correlation to histological type, clinical staging, gender and age, respectively. Results： ①The both two undifferentiated renal carcinoma showed evidence of increased or decreased DNA sequence copy numbers involving one or more regions of the genome. The frequently gained chromosome regions in URCC were lp,3q,2q,16q,1q, and the frequently lost chromosome regions were 17p 6p, 16p,20p, 17q,5p,3p,11q. ②Immunohistochemieal staining showed that CD10 ,CK ,VIM were positively exoressed in 2 cases of URCC, and P53 was strong positive. Conclusion： Gains of 1p,3q, 2q,16q,lq and losses of 17p,6p,16p,20p,17q,5p,3p,11q may be involved in the tumorigenesis of URCC. Losses of 17p may be correlated with advanced stage and poor prognosis of CRCC.

关 键 词：未分类肾癌 比较基因组杂交 

分 类 号：R73-3[医药卫生—肿瘤]