两种相干光断层扫描仪测量兔眼视网膜厚度和组织学检查的对比研究  

Comparability of sensory retinal thickness measured by different OCTs to histological studies in rabbit eyes

作　　者：黄丽娜[1] 樊宁[1] 成洪波[1] 赖铭莹[1] 赵军[1] 

机构地区：[1]暨南大学附属深圳市眼科医院,深圳518001

出　　处：《眼科》2009年第4期239-242,共4页Ophthalmology in China

基　　金：广东省科技计划项目(2005B10401049)

摘　　要：目的探讨兔眼视网膜厚度的国产与进口Stratus相干光断层扫描(OCT)仪检测与组织学检查值之间的相关性。设计实验研究。研究对象15只兔眼。方法先对兔眼的视网膜检测部位进行激光标记定位,分别用国产OSE-1800型OCT实验样机和Stratus OCT扫描并测量该部位的视网膜厚度值,再采用组织学方法检测相应部位的视网膜厚度值,应用SPSS 11.5软件统计学对比分析。主要指标视网膜神经上皮层厚度值。结果OSE-1800 OCT测量兔眼视网膜神经上皮层厚度平均值为(122.5±7.4)μm,Stratus OCT测量值为(118.0±5.6)μm,组织学测量值为(116.3±8.8)μm,P=0.292;OSE-1800 OCT与Stratus OCT测量兔眼视网膜神经上皮层厚度的相关系数为0.914(P=0.000),OSE-1800 OCT与组织学测量值的相关系数为0.872(P=0.001),OSE-1800 OCT与组织学测量值的相关系数为0.833(P=0.002)。结论OSE-1800型OCT与Stratus OCT均能准确地定量测量兔眼视网膜厚度,它们与组织形态学测量值均有较好的相关性。Objective To study correlation of the retinal nerve epithelium layer thickness measured with different optical coherence tomography （OCT） in vivo with histological measurement. Design Experimental study. Participants 15 rabbit eyes. Methods The retina measurement position of 15 rabbit eyes were marked by laser, and then were scanned by OSE-1800 OCT and Stratus OCT. Retinal nerve epithelium layer thickness was measured in retinal histological slides of rabbit eyes. The results measured with three methods were compared and linear regression analyses were done with SPSSll.5 software. Results The average retinal nerve epithelium layer thickness measured with OSE-1800 OCT, Stratus OCT and histological method were 119.5±7.4, 118.0±5.6, and 116.3±8.8μm respectively（P=0.292）. Retinal nerve epithelium layer thickness measured with both OCT instruments had the best correlation （r=0.914, P= 0.000）, and the thickness measured with Stratus OCT and histological method had the better correlation （r=0.872, P=0.001）, and the thickness measured with OSE-1800 OCT and histological method had the significant correlation （r=0.833, P=0.002）. Conclusions The retinal nerve epithelium layer thickness measured with different OCTs in vivo correlate well with histomorphometry, and the measurement of both OCT instruments are accurate. （Ophthalmol CHN, 2009, 18： 239-242）

关 键 词：相干光断层扫描 视网膜 实验研究 

分 类 号：R774.1[医药卫生—眼科]