人高度近视眼房水中基质金属蛋白酶-2的表达  

Expression of MMP-2 in aqueous humor from patients with high myopia

作　　者：陶靖[1] 刘谦[1] 赵博文[1] 王宁利[1] 王军[1] 

机构地区：[1]首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科学与视觉科学重点实验室,100730

出　　处：《眼科》2009年第4期260-264,共5页Ophthalmology in China

基　　金：国家自然科学基金资助项目(30700920);北京市科技新星计划(B类)资助项目(2005B50)

摘　　要：目的比较高度近视及正视的白内障患者房水基质金属蛋白酶-2(MMP-2)的表达情况,探讨房水中MMP-2与高度近视的相关性。设计实验研究。研究对象30例年龄相关性白内障患者的房水。方法白内障手术中收集合并高度近视患者房水15例(实验组)及正视眼患者房水15例(对照组)。将房水标本通过蛋白质印迹法检测MMP-2酶原和活化MMP-2的表达。主要指标房水MMP-2的表达灰度值。结果实验组中MMP-2酶原表达量(430.4±57.3)大于活化MMP-2(294.5±35.2)(t=10.400,P=0.000);对照组中MMP-2酶原表达量(402.8±57.7)大于活化MMP-2(280.3±49.7)(t=8.400,P=0.000)。实验组和对照组间比较,MMP-2酶原(t=1.320,P=0.200)及活化MMP-2(t=0.900,P=0.375)表达量差异均无统计学意义。结论在本样本有限的研究中,未检测到高度近视眼患者房水中MMP-2表达量的异常,房水中MMP-2有少量以活化形式存在,大部分以酶原形式存在,具有应激活化的潜能。Objective To investigate the correlation between high myopia and MMP-2 in aqueous humor （AH）, by comparing the protein level of MMP-2 in AH from patients with cataract combined with high myopia and with emmetropia. Design Experimental study. Participants 30 AH samples of patients with age-related cataract. Methods AH samples were collected from the patients with age-related cataract during phacoemulsification, including 15 AH samples from patients combined with high myopia （experimental group） and 15 AH samples from patients with emmetropia （control group）. The expression of pro-MMP-2 and MMP-2 in AH were analyzed by Western blot technique. Main Outcome Measures The gray value of MMP-2 protein band detected by Western blot. Results The level of pro MMP-2 was statistically higher than that of MMP-2 in experimental group （430.4±57.3 versus 294.5±35.2, t=10.400, P= 0.000） and control group （402.8±57.7 versus 280.3±49.7, t=8.400, P=0.000）. There was no statistically difference in the level of pro-MMP-2（t=1.320, P=0.200）and MMP-2 （t=0.900, P=0.375） between the two groups. Conclusions No abnormal expression of MMP-2 was detected in AH from patients with high myopia, according to this study based on limited samples. Pro-MMP-2 was the main form of MMP-2 in AH of patients with cataract combined with high myopia or emmetropia, which possessed potential ability of transferring into form of active MMP-2. （Ophthalmol CHN, 2009, 18： 260-264）

关 键 词：高度近视跟 基质金属蛋白酶-2 房水 

分 类 号：R778.1[医药卫生—眼科]