脊髓小脑共济性失调2型家系永生细胞株的建立及遗传稳定性检测  

Establishment of immortalized cell lines and genetic stability evaluation for a family with spinocerebellar ataxia type 2

作　　者：林克勤[1] 孙浩[1] 张昌军[2] 陶玉芬[1] 易文[1] 黄小琴[1] 史荔[1] 褚嘉祐[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所,昆明650118 [2]湖北省十堰市人民医院生殖医学中心

出　　处：《中华医学遗传学杂志》2009年第4期374-378,共5页Chinese Journal of Medical Genetics

摘　　要：目的建立伴帕金森氏病症状的脊髓小脑共济性失调2型家系永生细胞株，提供永久性的实验研究材料。方法采用EB病毒加入环胞霉素A的转化细胞技术，建立了该家系的永生细胞株。并检测传代细胞与家系血液中的微卫星标记有无改变，确定细胞株的遗传稳定性。结果成功建立此家系25株永生细胞株，所检测的微卫星位点未发生变化。结论所建永生细胞株染色体核型检测无异常，转化细胞株与血液中的微卫星的遗传稳定性无差异。Objective Immortalized cell lines of spinocerebellar ataxia type 2 （SCA2） with Parkinson disease symptoms were established in order to provide experimental material for future study. Methods The immortalized cell lines were constructed by using Epstein Barr virus and cyclosporine A. Microsatellite markers were detected to see whether there is any change between the cell lines and the original blood samples, and the genetic stability of the cell lines were evaluated. Results Twenty-five immortalized cell lines were established successfully from the family and the microsatellite markers were unchanged. Conclusion The karyotypes of the immortal cell lines were normal and the cell lines were genetically stable.

关 键 词：EB病毒 环胞霉素A 永生细胞株 伴帕金森氏病症状的脊髓小脑共济性失调2型 微卫星 遗传稳定性 

分 类 号：R686[医药卫生—骨科学]