抗Ro-52抗体在自身免疫性肝病中的检测  

Antibody to Ro-52 in patients with autoimmune liver disease

作　　者：赵丹彤[1] 闫惠平[1] 檀玉芬[1] 刘妍[1] 赵艳[1] 冯霞[1] 向代军[1] 

机构地区：[1]首都医科大学附属北京佑安医院自身免疫性肝病临床研究中心,100069

出　　处：《中华微生物学和免疫学杂志》2009年第7期656-659,共4页Chinese Journal of Microbiology and Immunology

基　　金：北京市自然科学基金资助项目（7092046）

摘　　要：目的探讨抗Ro-52抗体对自身免疫性肝病（autoimmune liver disease，AILD）的临床意义。方法对采用免疫印迹法检测抗Ro-52抗体的115例AILD患者的临床资料进行回顾性分析，比较抗Ro-52阳性和阴性AILD患者肝功和免疫学指标，对可能有相关性的血清学指标进行诊断试验一致性评价。结果抗Ro-52抗体在自身免疫性肝炎（autoimmune hepatitis，AIH）（37例）、原发性胆汁性肝硬化（primary biliary cirrhosis，PBC）（57例）、AIH／PBC重叠综合征组（21例）的阳性率分别为32．43％、24．56％、33．33％，差异无统计学意义（χ^2=0．949，P〉0．05）。抗可溶性肝抗原／肝胰抗原抗体（anti—soluble liver antigen／liver—pancreas，anti—SLA／LP）在抗Ro-52阳性AIH组频率（58．33％）高于阴性组（16．00％）（χ^2=6．955，P〈0．05），抗SLA／LP抗体在抗Ro-52阳性AIH／PBC重叠综合征组频率（85．71％）高于阴性组（28．57％）（χ^2=6．109，P〈0．05）。抗Ro-52抗体和抗SLA／LP抗体结果有一致性（κ=0．466，P〈0．05）。AIH／PBC重叠综合征组抗Ro-52阳性患者IgG水平高于阴性患者（t=2．508，P〈0．05）。结论抗Ro-52抗体在AIH、PBC和AIH／PBC重叠综合征中的分布没有差别；抗Ro-52抗体与抗SLA／LP抗体检测结果有一致性；抗Ro-52抗体阳性AIH／PBC重叠综合征患者IgG水平高于抗体阴性者。Objective To investigate the significance of antibody to Ro-52 in patients with autoimmune liver disease （AILD）. Methods One hundred and fifteen patients with abnormal liver functions, who had anti-Ro-52 detection by immunological blotting, were reviewed retrospectively. According to types of AILD, the clinical features were compared between patients with and without anti-Ro-52, respectively. K test of concordance was used to provide a chance-corrected valve for immune-serological results. Results The rates of anti-Ro-52 in autoimmune hepatitis （AIH）, primary sclerosing cholangitis （PBC） and AIH/PBC overlap syndrome groups were 32.43% , 24.56% and 33.33%, respectively, there were no significant differences among three groups （ χ^2 = 0.949, P 〉0. 05 ）. The rate of anti-soluble liver antigen/liver-pancreas （ anti-SLA/LP） in AIH patients with anti-Ro-52 （58.33%） was higher than AIH patients without anti-Ro-52 （ 16.00% ,P 〈 0.05 ）. The rate of anti-SLA/LP in AIH/PBC overlap syndrome patients with anti-Ro-52 （85.71% ） was also higher than that of control group （28.57%, P 〈 0.05 ）. Anti-Ro-52 and anti-SLA/LP had concordance according to K test（ K 〉 0.40, P 〈 0.05 ）. The average level of IgG in AIH/PBC overlap syndrome patients with anti-Ro-52 was higher than patients without anti-Ro-52 （ t = 2. 508, P 〈 0.05 ）. Conclusion The rates of anti-Ro-52 in AIH, PBC and AIH/PBC overlap syndrome were of no significant differences. Anti-Ro-52 may have correlation with anti-SLA/LP. AIH/PBC overlap syndrome patients with anti-Ro-52 showed higher IgG level than patients without anti-Ro-52.

关 键 词：肝病 自身免疫性 自身抗体 抗Ro-52抗体 

分 类 号：R575[医药卫生—消化系统] R446.62[医药卫生—内科学]